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Invitrogen
AHR Monoclonal Antibody (RPT1)
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FIGURE: 1 / 46
AHR Antibody (MA1-514) in ICC/IF
Immunofluorescence analysis of AHR was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with AHR Monoclonal Antibody (RPT1) (Product # MA1-514) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175... View More
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.jpg?time=20220826?time=20220826 "AHR Antibody (MA1-514) in WB")
.jpg?time=20220826?time=20220826 "AHR Antibody (MA1-514) in WB")
Product Details
MA1-514
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse,
Non-human primate,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
RPT1
Immunogen
Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of human AhR.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-514 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.
MA1-514 has been successfully used in Western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~96 kDa protein representing AhR, with some minor non-specific bands observed in certain samples.
MA1-514 immunogen is residues 12-31 from the human AhR protein with amino acids 18-21 being omitted.
Target Information
AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ah receptor; AH-receptor; aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76; dioxin receptor
Gene Aliases: Ah; Ahh; AHR; Ahre; BHLHE76; In
UniProt ID: (Human) P35869, (Mouse) P30561
Entrez Gene ID: (Human) 196, (Mouse) 11622, (Rat) 25690
DNA binding
transcription factor activity, sequence-specific DNA binding
aryl hydrocarbon receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
transcription factor binding
enhancer binding
transcription regulatory region DNA binding
protein heterodimerization activity
protein dimerization activity
Hsp90 protein binding
E-box binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
signal transducer activity
sequence-specific DNA binding
basic helix-loop-helix transcription factor
ovarian follicle development
blood vessel development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
xenobiotic metabolic process
apoptotic process
cell cycle
response to xenobiotic stimulus
response to toxic substance
regulation of gene expression
intracellular receptor signaling pathway
regulation of B cell proliferation
response to estradiol
circadian regulation of gene expression
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to glucocorticoid stimulus
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
patterning of blood vessels
B cell homeostasis
liver development
B-1 B cell homeostasis
blood vessel remodeling
lymphocyte homeostasis
immune system process
negative regulation of systemic arterial blood pressure
cardiac left ventricle morphogenesis
circumferential growth involved in left ventricle morphogenesis
transcription, DNA-templated
response to stress
signal transduction
blood circulation
regulation of blood pressure
response to organic cyclic compound
B cell differentiation
prostate gland development
embryonic hemopoiesis
post-embryonic hemopoiesis
camera-type eye development
T cell homeostasis
positive regulation of cell size
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
negative regulation of vasoconstriction
positive regulation of growth
rhythmic process
blood vessel morphogenesis
spleen development
reproductive structure development
gland development
smooth muscle tissue development
regulation of blood vessel size
regulation of heart growth
negative regulation of necrotic cell death
venous blood vessel development
kidney morphogenesis
common bile duct development
cellular response to cAMP
glomerulus morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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