Invitrogen

AHR Monoclonal Antibody (RPT9)

Name: AHR Monoclonal Antibody (RPT9)
Brand: Invitrogen
SKU: MA1-513

Advanced Verification

19 Published Figures

43 References

View all (45) AHR antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

AHR Antibody in Immunocytochemistry (ICC/IF)

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AHR Antibody (MA1-513) in ICC/IF

Immunofluorescent analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) (Product # MA1-513) shows staining in A2058 Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor (Product # MA1-513) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated... View More

AHR Antibody in Immunohistochemistry (IHC)

AHR Antibody in Western Blot, Immunohistochemistry (WB, IHC)

AHR Antibody in Western Blot, Immunoprecipitation (WB, IP)

AHR Antibody in Immunoprecipitation (IP)

Product Image Gallery

AHR Antibody — Fig. 5 Total amounts of endogenous AhR in the cytosol and nucleus of HeLa cells. (A) HeLa cells were treated with 0.05% DMSO (-) or 1 muM beta-NF (+) for 0, 15, 30, 60, and 120 min at 37 degC. The lysate was collected and centrifuged for 60 min at 105,000 g , then the supernatant was collected. AhR in the cytosol were analyzed by immnoblotting with an anti-AhR antibody or an anti- beta-actin antibody ( n = 4, * p < 0.05). The AhR/beta-actin ratio was quantified using Image J software (Drop of Wisdom). (B) HeLa cells were treated with 0.05% DMSO (-) or 3 muM beta-NF (+) for 30 min at 37 degC. The lysate was collected and centrifuged, then the supernatant was collected. Immunoprecipitation was performed using an anti-Ahr antibody or anti-HSP90 antibody followed by immunoblotting with an anti-HSP90 antibody or an anti-AhR antibody. In panel, the HeLa cell lysate (left two lanes) or immunoprecipitated samples (right two lanes) were separated by SDS-PAGE followed by immnoblotting. The subunit molecular weight of HSP90 and AhR are 90 and 110 kDa, respectively. The 50- and 32-kDa protein bands were the IgG heavy- and light chain, respectively. M, molecular marker proteins. C, HeLa cells were treated with 0.05% DMSO (-) or 1 muM beta-NF (+) for 0, 15, 30, 60, 120, and 360 min at 37 degC. The lysate was collected and centrifuged for 60 min at 105,000 g , then the supernatant was collected as a cytosol fraction. Or the lysate in 2.2-M sucrose solution was centrifuged for 60 min at 30,0 {TM}

Immunogen image

Immunogen Image AHR Monoclonal Antibody (RPT9)

Product Details

MA1-513

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

View 5 publications 5 publications

Flow Cytometry (Flow)

1:50

View 1 publication 1 publication

ELISA (ELISA)

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

ChIP assay (ChIP)

1:100

View 7 publications 7 publications

Gel Shift (EMSA)

Assay-dependent

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

C. elegans, Human, Mouse, Rat

Host/Isotype

Mouse / IgG3, kappa

Class

Monoclonal

Type

Antibody

Clone

RPT9

Immunogen

Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2223958

Product Specific Information

MA1-513 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.

MA1-513 has been successfully used in immunoprecipitation, chromatin immunoprecipitation, immunocytochemistry, immunohistochemistry, immunofluorescence, and gel shift procedures. This antibody specifically immunoprecipitates a single ~95 kDa protein representing AhR from Hepa 1 cytosol. Although MA1-513 can be used in Western blotting procedures, MA1-514 (clone RPT1) is recommended for this procedure.

The MA1-513 immunogen is a synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.

Target Information

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Ah receptor; AH-receptor; aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76; dioxin receptor

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Gene Aliases: Ah; Ahh; AHR; Ahre; BHLHE76; In

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UniProt ID: (Human) P35869, (Mouse) P30561

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Entrez Gene ID: (Human) 196, (Mouse) 11622, (Rat) 25690

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding aryl hydrocarbon receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding enhancer binding transcription regulatory region DNA binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding E-box binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding signal transducer activity sequence-specific DNA binding basic helix-loop-helix transcription factor

Process(es)

ovarian follicle development blood vessel development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter xenobiotic metabolic process apoptotic process cell cycle response to xenobiotic stimulus response to toxic substance regulation of gene expression intracellular receptor signaling pathway regulation of B cell proliferation response to estradiol circadian regulation of gene expression negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cellular response to glucocorticoid stimulus negative regulation of transcription from RNA polymerase II promoter cell morphogenesis patterning of blood vessels B cell homeostasis liver development B-1 B cell homeostasis blood vessel remodeling lymphocyte homeostasis immune system process negative regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis circumferential growth involved in left ventricle morphogenesis transcription, DNA-templated response to stress signal transduction blood circulation regulation of blood pressure response to organic cyclic compound B cell differentiation prostate gland development embryonic hemopoiesis post-embryonic hemopoiesis camera-type eye development T cell homeostasis positive regulation of cell size positive regulation of RNA polymerase II transcriptional preinitiation complex assembly negative regulation of vasoconstriction positive regulation of growth rhythmic process blood vessel morphogenesis spleen development reproductive structure development gland development smooth muscle tissue development regulation of blood vessel size regulation of heart growth negative regulation of necrotic cell death venous blood vessel development kidney morphogenesis common bile duct development cellular response to cAMP glomerulus morphogenesis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AHR Monoclonal Antibody (RPT9)

AHR Antibody (MA1-513) in ICC/IF

Product Details

MA1-513

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (EMSA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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