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Invitrogen
IGF1R alpha Monoclonal Antibody (24-31)
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FIGURE: 1 / 5
IGF1R alpha Antibody (AHR0321) in ICC/IF
Immunofluorescent analysis of IGF-1 Receptor alpha (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an IGF-1 Receptor alpha monoclonal antibody (Product # AH... View More
Product Details
AHR0321
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
24-31
Immunogen
IGF-1R/3T3 mouse fibroblasts transfected with human type 1 IGF-receptor cDNA.
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with an epitope at aa 283-440 (exon 4-6).
Recommended positive controls include placenta or breast tissue. This antibody is not suitable for Western blot. Without BSA, this antibody can be used as a capture antibody in a sandwich ELISA.
Target Information
IGF1R (Insulin-like Growth Factor1 Receptor) is a receptor tyrosine kinase that mediates actions of insulin-like growth factor 1 (IFG1). It binds IGF1 with high affinity and IGF2/insulin with lower affinity. Activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survial of malignant cells. Ligand binding activates the receptor kinase and leads to receptor autophosphorylation and tyrosines phosphorylation of multiple substrates that function as signaling adapter proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD221; IGF-I receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor
Gene Aliases: CD221; IGF1R; IGFIR; IGFR; JTK13
UniProt ID: (Human) P08069
Entrez Gene ID: (Human) 3480
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
insulin-activated receptor activity
insulin-like growth factor-activated receptor activity
insulin receptor binding
structural molecule activity
protein binding
insulin-like growth factor binding
ATP binding
kinase activity
transferase activity
insulin-like growth factor I binding
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
protein transporter activity
immune response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of cell migration
peptidyl-tyrosine autophosphorylation
negative regulation of apoptotic process
positive regulation of protein complex disassembly
negative regulation of MAPK cascade
positive regulation of MAPK cascade
protein kinase B signaling
transcytosis
regulation of JNK cascade
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
positive regulation of protein kinase B signaling
cellular response to glucose stimulus
dendritic spine maintenance
beta-amyloid clearance
positive regulation of cold-induced thermogenesis
cellular response to oxygen-containing compound
cellular response to beta-amyloid
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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