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Invitrogen
AIDA Polyclonal Antibody
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FIGURE: 1 / 2
AIDA Antibody (PA5-143932) in WB
Western blot analysis of AIDA using AIDA antibody (Product # PA5-143932). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human 293T whole cell lysates; Lane 2: human U251 whole cell lysates; Lane 3: human PC-3 whole cell lysates; Lane 4: human U-87MG whole cell lysates; Lane 5: rat stomach tissue lysates; Lane 6: mouse RAW264. 7 whole cell lysates; Lane 7: mouse NIH/3T3 whole cell... View More
Product Details
PA5-143932
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human AIDA recombinant protein (Position: E54-R284).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, human U251 whole cell, human PC-3 whole cell, human U-87MG whole cell, rat stomach tissue, mouse RAW2647 whole cell, mouse NIH/3T3 whole cell. Flow: K562 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
AIDA acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Axin interaction partner and dorsalization antagonist; Axin interactor, dorsalization-associated protein; unnamed protein product
Gene Aliases: 2610208M17Rik; AIDA; BC004835; C1orf80
UniProt ID: (Human) Q96BJ3, (Mouse) Q8C4Q6
Entrez Gene ID: (Human) 64853, (Mouse) 108909, (Rat) 682999
multicellular organism development
dorsal/ventral pattern formation
negative regulation of Wnt signaling pathway
regulation of protein homodimerization activity
negative regulation of JUN kinase activity
negative regulation of JNK cascade
determination of ventral identity
negative regulation of determination of dorsal identity
negative regulation of protein complex assembly
regulation of protein complex assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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