Cell lysates prepared from Jurkat cells (lane 1) and mouse macrophages left untreated (lane 2) or treated for 12 hr with IFN gamma and LPS (lane 3) were immunoblotted with a 1:1000 dilution of the Anti-AIM2 polyclonal antibody. Bands were visualized using Anti-Rabbit IgG HRP.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
Description: This polyclonal antibody reacts with human, mouse, and rat AIM2 (also known as Antigen isolated from Immunoselected Melanoma 2). A member of the HIN-2000 family, this 40-kDa cytoplasmic protein is expressed in the spleen, small intestine, peripheral blood lymphocytes, and testis. Moreover, AIM2 is expressed in tumors of the neuroectodermis, breast, ovary, and colon. As a component of the inflammosome, AIM2 detects double-stranded viral and bacterial DNA present in the cytosol and initiates Caspase1 activation. Expression of AIM2 can be induced by interferons.
Applications Reported: This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested: This polyclonal antibody has been tested by immunoblotting of Jurkat cells and mouse macrophages treated for 12 hrs with IFN gamma and LPS. This can be used at less than or equal to a 1:1000 dilution. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||The AIM2 inflammasome is critical for innate immunity to Francisella tularensis.||Fernandes-Alnemri T,Yu JW,Juliana C,Solorzano L,Kang S,Wu J,Datta P,McCormick M,Huang L,McDermott E,Eisenlohr L,Landel CP,Alnemri ES||Nature immunology (11:385)||2010|
|Not Applicable||Not Cited||AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC.||Hornung V,Ablasser A,Charrel-Dennis M,Bauernfeind F,Horvath G,Caffrey DR,Latz E,Fitzgerald KA||Nature (458:514)||2009|