Search
Invitrogen
AIM2 Polyclonal Antibody, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 15
AIM2 Antibody (14-6008-93) in WB
Cell lysates prepared from Jurkat cells (lane 1) and mouse macrophages left untreated (lane 2) or treated for 12 hr with IFN gamma and LPS (lane 3) were immunoblotted with a 1:1000 dilution of the Anti-AIM2 polyclonal antibody. Bands were visualized using Anti-Rabbit IgG HRP.
Product Details
14-6008-93
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol, 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This polyclonal antibody reacts with human, mouse, and rat AIM2 (also known as Antigen isolated from Immunoselected Melanoma 2). A member of the HIN-2000 family, this 40-kDa cytoplasmic protein is expressed in the spleen, small intestine, peripheral blood lymphocytes, and testis. Moreover, AIM2 is expressed in tumors of the neuroectodermis, breast, ovary, and colon. As a component of the inflammosome, AIM2 detects double-stranded viral and bacterial DNA present in the cytosol and initiates Caspase1 activation. Expression of AIM2 can be induced by interferons.
Applications Reported: This polyclonal antibody has been reported for use in immunoblotting (WB).
Applications Tested: This polyclonal antibody has been tested by immunoblotting of Jurkat cells and mouse macrophages treated for 12 hrs with IFN gamma and LPS. This can be used at less than or equal to a 1:1000 dilution. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Absent in melanoma 2; Ifi-210; Interferon-inducible protein 210; Interferon-inducible protein AIM2; Interferon-inducible protein p210; RP11-520H16.3; unnamed protein product
Gene Aliases: AIM2; Gm1313; Ifi210; PYHIN4
UniProt ID: (Human) O14862, (Mouse) Q91VJ1
Entrez Gene ID: (Human) 9447, (Rat) 304987, (Mouse) 383619
activation of innate immune response
pattern recognition receptor signaling pathway
positive regulation of defense response to virus by host
immune system process
inflammatory response
immune response
cellular response to DNA damage stimulus
brain development
negative regulation of NF-kappaB transcription factor activity
positive regulation of interleukin-1 beta production
tumor necrosis factor-mediated signaling pathway
cellular response to interferon-beta
T cell homeostasis
innate immune response
positive regulation of inflammatory response
regulation of behavior
positive regulation of NF-kappaB transcription factor activity
protein maturation
defense response to virus
negative regulation of protein kinase B signaling
neuron cellular homeostasis
pyroptosis
cellular response to cytokine stimulus
cellular response to xenobiotic stimulus
AIM2 inflammasome complex assembly
pyroptotic cell death
pyroptosome complex assembly
biological_process
positive regulation of protein oligomerization
cellular response to drug
interleukin-1 beta secretion
positive regulation of interleukin-1 beta secretion
positive regulation of cysteine-type endopeptidase activity
apoptotic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support