Proteintech

AKAP1 Polyclonal Antibody

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AKAP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using AKAP1 antibody 15618-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

AKAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 15618-1-AP (AKAP1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

AKAP1 Antibody in Western Blot (WB) — Human liver tissue were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

AKAP1 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug AKAP1 antibody (15618-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

AKAP1 Antibody in Immunoprecipitation (IP) — IP result of anti-AKAP1 (IP:15618-1-AP, 4ug; Detection:15618-1-AP 1:2000) with PC-3 cells lysate 1800ug.

Product Details

15618-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AKAP1 Fusion Protein Ag8037 (31-351 aa encoded by BC000729)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: GHVSSHDEQ QVEAGAVQLR ADPAIKEPLP VEDVCPKVVS TPPSVTEPPE KELSTVSKLP AEPPALLQTH PPCRRSESSG ILPNTTDMRL RPGTRRDDST KLELALTGGE AKSIPLECPL SSPKGVLFSS KSAEVCKQDS PFSRVPRKVQ PGYPVVPAEK RSSGERARET GGAEGTGDAV LGEKVLEEAL LSREHVLELE NSKGPSLASL EGEEDKGKSS SSQVVGPVQE EEYVAEKLPS RFIESAHTEL AKDDAAPAPP VADAKAQDRG VEGELGNEES LDRNEEGLDR NEEGLDRNEE SLDRNEEGLD RNEEIKRAAF QI (31-351 aa encoded by BC000729)

Target Information

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

The EMBO journal

Wu W,Lin C,Wu K,Jiang L,Wang X,Li W,Zhuang H,Zhang X,Chen H,Li S,Yang Y,Lu Y,Wang J,Zhu R,Zhang L,Sui S,Tan N,Zhao B,Zhang J,Li L,Feng D

Fri Jul 01 00:00:00 EDT 2016

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

The EMBO journal

Wu W,Lin C,Wu K,Jiang L,Wang X,Li W,Zhuang H,Zhang X,Chen H,Li S,Yang Y,Lu Y,Wang J,Zhu R,Zhang L,Sui S,Tan N,Zhao B,Zhang J,Li L,Feng D

Fri Jul 01 00:00:00 EDT 2016

Short RNA molecules with high binding affinity to the KH motif of A-kinase anchoring protein 1 (AKAP1): implications for the regulation of steroidogenesis.