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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000-1:6,000 | View 3 publications 3 publications |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
Immunoprecipitation (IP) |
Assay-dependent | View 1 publication 1 publication |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
AKAP3 Fusion Protein Ag4885 (1-300 aa encoded by BC047535) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.49 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MSEKVDWLQ SQNGVCKVDV YSPGDNQAQD WKMDTSTDPV RVLSWLRRDL EKSTAEFQDV RFKPGESFGG ETSNSGDPHK GFSVDYYNTT TKGTPERLHF EMTHKEIPCQ GPRAQLGNES SVDEVSFYAN RLTNLVIAMA RKEINEKIDG SENKCVYQSL YMGNEPTPTK SLSKIASELV NETVSACSRN AAPDKAPGSG DRVSGSSQSP PNLKYKSTLK IKESTKERQG PDDKPPSKKS FFYKEVFESR NGDYAREGGR FFPRERKRFR GQERPDDFTA SVSEGIMTYA NSVVSDMMVS I (1-300 aa encoded by BC047535)
The type II cAMP-dependent protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins, designated A-kinase anchoring proteins (AKAP). AKAP 3, also known as AKAP 110, FSP95, PRKA3 and SOB1, binds both PKA and PDE4A and functions as a scaffolding protein in spermatozoa to regulate local cAMP concentrations and modulate sperm functions. Expression of AKAP 3 in normal tissues is restricted to the testis, where bicarbonate stimulates tyrosine phosphorylation of AKAP 3, thereby increasing its recruitment of PKA. AKAP-3 also exhibits high expression in patients with epithelial ovarian cancer (EOC). It demonstrates tumor-restricted expression and appears to be associated with worse overall survival, which make AKAP 3 a potential target for antigen-specific immunotherapy in EOC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: A kinase (PRKA) anchor protein 3; A-kinase anchor protein 110 kDa; A-kinase anchor protein 3; A-kinase anchor protein, 110kDa; AKAP 110; AKAP-3; Cancer/testis antigen 82; CT82; epididymis luminal protein 159; Fibrous Sheath Protein of 95 kDa; fibrousheathin 1; Fibrousheathin I; Fibrousheathin-1; FSP95; PRKA3; protein kinase A binding protein AKAP 110; Protein kinase A-anchoring protein 3; Sperm oocyte-binding protein; sperm oocyte-binding protein 1
Gene Aliases: AKAP 110; AKAP110; AKAP3; CT82; FSP95; HEL159; PRKA3; SOB1
UniProt ID: (Human) O75969, (Mouse) O88987
Entrez Gene ID: (Human) 10566, (Mouse) 11642, (Rat) 312720
Molecular Function:
scaffold/adaptor protein
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