|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||17 amino acid peptide near the amino terminus of human AKAP4|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||20 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A recommended positive control is human testis tissue lysate.
At least three isoforms are known to exist; this antibody will detect the two longest isoforms.
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. AKAP4, also known as hAKAP82, is a major sperm fibrous sheath protein that localizes to the entire length of the flagellum of human sperm. It has been suggested that AKAP4 plays multiple roles in sperm motility and regulation of signal transduction pathways. AKAP4 has also been postulated to be a potential biomarker for breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.