Invitrogen

AKAP8L Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (11) AKAP8L antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

AKAP8L Antibody in Immunofluorescence (IF) — Immunofluorescent staining of AKAP8L in human cell line MCF7 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using an AKAP8L Polyclonal Antibody (Product # PA5-59993).

AKAP8L Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of AKAP8L in human cerebral cortex, colon, kidney and testis using AKAP8L Polyclonal Antibody (Product # PA5-59993) (A) shows similar protein distribution across tissues to an independent AKAP8L Polyclonal Antibody (B).

AKAP8L Antibody in Western Blot (WB) — Western blot analysis of AKAP8L in human cell line RT-4 and human cell line U-251 MG using a AKAP8L Polyclonal Antibody (Product # PA5-59993).

AKAP8L Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of AKAP8L demonstrates antibody specificity. Immunohistochemical analysis of AKAP8L using anti-AKAP8L Polyclonal Antibody (Product # PA5-59993), shows significant staining of AKAP8L in human testis and shows minimal or weak staining in human liver tissues. The relative expression levels of AKAP8L within each tissue is shown using RNA-Seq. {RE}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1-4 µg/mL

Immunofluorescence (IF)

1-4 µg/mL

Immunohistochemistry (IHC)

1:200-1:500

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human AKAP8L

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2637817

Product Specific Information

Immunogen sequence: DEEGKEDGRE EGKEDPEKGA LTTQDENGQT KRKLQAGKKS QDKQKKRQRD RMVERIQFVC SLCKYRTFYE DEMASHLDSK FHKEHFKYVG TKLPKQTAD

Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 93%.

Target Information

Could play a role in constitutive transport element (CTE)-mediated gene expression. Does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May regulate the initiation phase of DNA replication when associated with TMPO-beta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: A kinase (PRKA) anchor protein 8-like; A-kinase anchor protein 8-like; AKAP8-like protein; HA95; HAP95; Helicase A-binding protein 95; helicase A-binding protein 95 kDa; Homologous to AKAP95 protein; NAKAP; NAKAP95; neighbor of A kinase anchoring protein 95; neighbor of A-kinase anchoring protein 95; Neighbor of A-kinase-anchoring protein 95; neighbor of AKAP; Neighbor of AKAP95; testis tissue sperm-binding protein Li 90mP

Gene Aliases: AKAP8L; HA95; HAP95; HRIHFB2018; NAKAP; NAKAP95

UniProt ID: (Human) Q9ULX6

Entrez Gene ID: (Human) 26993

Function(s)