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Invitrogen
ALAS1 Polyclonal Antibody
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FIGURE: 1 / 3
ALAS1 Antibody (PA1-41152) in IHC (P)
Immunohistochemistry analysis of ALAS1 in human skin. Samples were incubated with ALAS1 polyclonal antibody (Product # PA1-41152) using a dilution of 5 µg/mL. Heat induced antigen retrieval in pH 6.0 citrate buffer was used for unmasking. A biotinylated secondary antibody followed by alkaline phosphatase-streptavidin was and chromogen was used for detection.
Product Details
PA1-41152
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A portion of amino acids 480-530 of human ALAS-1.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: HepG2.
Immunogen sequence homology: 100% homologous in human, chimp and beluga whale; 94% in dog and chicken; 88% in mouse, rat and lungfish; 83% in bovine, xenopus, pufferfish; 77% in coelacanth.
Target Information
ALAS-1 is a nuclear-encoded mitochondrial matrix enzyme which catalyzes the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-aminolevulinate synthase 1; 5-aminolevulinate synthase, non-specific, mitochondrial; 5-aminolevulinate synthase, nonspecific, mitochondrial; 5-aminolevulinic acid synthase 1; ALA-S; ALAS-H; ALAS-N; aminolevulinate, delta-, synthase 1; aminolevulinic acid synthase 1; Delta-ALA synthase 1; delta-ALA synthetase; Delta-aminolevulinate synthase 1; MIG4; migration-inducing protein 4; unnamed protein product
Gene Aliases: ALAS; ALAS-H; ALAS1; ALAS3; ALASH; MIG4; OK/SW-cl.121
UniProt ID: (Human) P13196, (Rat) P13195
Entrez Gene ID: (Human) 211, (Rat) 65155
response to hypoxia
porphyrin-containing compound metabolic process
protoporphyrinogen IX biosynthetic process
heme biosynthetic process
heme a biosynthetic process
heme B biosynthetic process
response to xenobiotic stimulus
response to herbicide
response to nickel cation
response to nutrient levels
response to cobalt ion
cellular response to insulin stimulus
tetrapyrrole biosynthetic process
response to gonadotropin
hemoglobin biosynthetic process
response to ethanol
heme O biosynthetic process
erythrocyte development
response to cAMP
response to platinum ion
response to bile acid
response to organic substance
response to organic cyclic compound
response to drug
cellular response to organic cyclic compound
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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