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Invitrogen
ALAS1 Polyclonal Antibody
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Product Details
PA5-29966
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ALAS-H. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.71 mg/mL
Amount
71 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Raji.
Predicted reactivity: Mouse (87%), Rat (85%), Bovine (85%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ALAS-1 is a nuclear-encoded mitochondrial matrix enzyme which catalyzes the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-aminolevulinate synthase, non-specific, mitochondrial; 5-aminolevulinate synthase, nonspecific, mitochondrial; 5-aminolevulinic acid synthase 1; ALAS-H; aminolevulinate, delta-, synthase 1; Delta-ALA synthase 1; Delta-aminolevulinate synthase 1; MIG4; migration-inducing protein 4; unnamed protein product
Gene Aliases: ALAS; ALAS-H; ALAS1; ALAS3; ALASH; MIG4; OK/SW-cl.121
UniProt ID: (Human) P13196
Entrez Gene ID: (Human) 211
response to hypoxia
porphyrin-containing compound metabolic process
protoporphyrinogen IX biosynthetic process
heme biosynthetic process
heme a biosynthetic process
heme B biosynthetic process
response to xenobiotic stimulus
response to herbicide
response to nickel cation
response to nutrient levels
response to cobalt ion
cellular response to insulin stimulus
tetrapyrrole biosynthetic process
response to gonadotropin
hemoglobin biosynthetic process
response to ethanol
heme O biosynthetic process
erythrocyte development
response to cAMP
response to platinum ion
response to bile acid
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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