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ALAS2 Antibody (CF809959) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALAS2 (RC219079, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ALAS2. Positive lysates LY424966 (100 µg) and LC424966 (20 µg) can be purchased separately from OriGene.
Product Details
CF809959
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2D3
Immunogen
Human recombinant protein fragment corresponding to amino acids 139-287 of human ALAS2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
ALAS2 specifies an erythroid-specific mitochondrially located enzyme. It catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia.The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-aminolevulinate synthase, erythroid-specific, mitochondrial; 5-aminolevulinic acid synthase 2; ALAS-E; Aminolevulinate synthase 2, delta; aminolevulinate, delta-, synthase 2; aminolevulinic acid synthase 2, erythroid; Delta-ALA synthase 2; delta-ALA synthetase; Delta-aminolevulinate synthase 2; erythroid-specific ALAS; erythroid-specific delta-aminolevulinate synthase ALAS-E; PRO2399; RP5-884M20.1
Gene Aliases: ALAS; Alas-2; ALAS-E; ALAS2; ALASE; ANH1; ASB; SIDBA1; XLDPP; XLEPP; XLSA
UniProt ID: (Human) P22557, (Rat) Q63147, (Mouse) P08680
Entrez Gene ID: (Human) 212, (Rat) 25748, (Mouse) 11656
response to hypoxia
protoporphyrinogen IX biosynthetic process
heme biosynthetic process
cellular iron ion homeostasis
erythrocyte differentiation
oxygen homeostasis
hemoglobin biosynthetic process
female pregnancy
lactation
response to lead ion
response to drug
porphyrin-containing compound metabolic process
metabolic process
biosynthetic process
tetrapyrrole biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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