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Invitrogen
ALDH4A1 Recombinant Mouse Monoclonal Antibody (7E2-R)
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FIGURE: 1 / 6
ALDH4A1 Antibody (MA5-56491) in ICC/IF
Immunocytochemistry analysis of ALDH4A1 with HepG2 cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp). Next they were incubated with ALDH4A1 Recombinant Monoclonal Antibody (Product # MA5-56491)at a dilution of 1:100 in 1% BSA in PBST overnight at 4 &(8)451;. Followed by Goat Anti-Rabbit IgG H&L at a dilution of 1:1,000. DAPI was used to stain the cell nuclei (blue).
Product Details
MA5-56491
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7E2-R
Immunogen
Synthetic peptide within Human ALDH4A1 aa 514-563/563.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aldehyde dehydrogenase 4 family, member A1; Aldehyde dehydrogenase family 4 member A1; Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial; L-glutamate gamma-semialdehyde dehydrogenase; mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase; P5C dehydrogenase; RP11-128M10.1
Gene Aliases: A930035F14Rik; Ahd-1; Ahd1; ALDH4; ALDH4A1; Aldh5a1; E330022C09; P5CD; P5CDH; P5cdhl; P5cdhs; Ssdh1
UniProt ID: (Human) P30038, (Mouse) Q8CHT0
Entrez Gene ID: (Human) 8659, (Rat) 641316, (Mouse) 212647
1-pyrroline-5-carboxylate dehydrogenase activity
aldehyde dehydrogenase (NAD) activity
electron carrier activity
identical protein binding
structural molecule activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
oxidoreductase activity
dehydrogenase
oxidoreductase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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