Invitrogen

ALDH9A1 Polyclonal Antibody

View all (14) ALDH9A1 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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ALDH9A1 Antibody in Western Blot (WB) — Western Blot analysis of ALDH9A1 using ALDH9A1 Polyclonal Antibody (Product # PA5-112089) at a dilution of 1:500. Lane A: Hela whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 55 kDa.

ALDH9A1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of ALDH9A1 was performed on (Lane A) 0.5 mg Hela whole cell lysate using 1 µL of ALDH9A1 Polyclonal Antibody (Product # PA5-112089) at a dilution of 1:500, and 60 µg of Immunomagnetic beads Protein A/G. A Clean-Blot IP Detection Reagent (HRP) was used as a secondary antibody at a dilution of 1:1,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 55 kDa.

Product Details

PA5-112089

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunoprecipitation (IP)

0.2-1 µL/mg lysate

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to the center region of the Human ALDH9A1/ALDH9

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2866825

Target Information

ALDH9A1 belongs to the aldehyde dehydrogenase family of proteins. The protein has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. The enzyme catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid (GABA). This isozyme is a tetramer of identical 54-kD subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 4-trimethylaminobutyraldehyde dehydrogenase; 4-trimethylaminobutyraldehyde dehydrogenase, N-terminally processed; aldehyde dehydrogenase (NAD+); aldehyde dehydrogenase 9 family, member A1; Aldehyde dehydrogenase E3 isozyme; Aldehyde dehydrogenase family 9 member A1; Gamma-aminobutyraldehyde dehydrogenase; R-aminobutyraldehyde dehydrogenase; TMABA-DH

Gene Aliases: ALDH4; ALDH7; ALDH9; ALDH9A1; E3; TMABADH

UniProt ID: (Human) P49189

Entrez Gene ID: (Human) 223

Molecular Function: dehydrogenase oxidoreductase metabolite interconversion enzyme