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Product Details
12292-1-AP
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
ALKBH3 Fusion Protein Ag2982 (148-286 aa encoded by BC015155) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.29 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Product Specific Information
Immunogen sequence: MEPNPHWHP VLRTLKNRIE ENTGHTFNSL LCNLYRNEKD SVDWHSDDEP SLGRCPIIAS LSFGATRTFE MRKKPPPEEN GDYTYVERVK IPLDHGTLLI MEGATQADWQ HRVPKEYHSR EPRVNLTFRT VYPDPRGAPW (148-286 aa encoded by BC015155)
Target Information
Dioxygenase that repairs alkylated DNA containing 1- methyladenine (1meA) and 3-methylcytosine (3meC) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated 3mC within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AlkB homolog 3; alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase; alkB, alkylation repair homolog 3; Alkylated DNA repair protein alkB homolog 3; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; DEPC 1; DEPC-1; FLJ43614; hABH3; m MGC118790; mAbh3; MGC118792; MGC118793; MGC134125; Prostate cancer antigen 1; prostate cancer antigen-1; RP23-375N21.4
Gene Aliases: 1700108H04Rik; 1810020C19Rik; ABH3; ALKBH3; DEPC-1; DEPC1; hABH3; mABH3; PCA1
UniProt ID: (Human) Q96Q83, (Rat) Q5XIC8, (Mouse) Q8K1E6
Entrez Gene ID: (Human) 221120, (Rat) 362169, (Mouse) 69113
Molecular Function:
oxygenase
protein binding
ferrous iron binding
L-ascorbic acid binding
DNA-N1-methyladenine dioxygenase activity
cytosine C-5 DNA demethylase activity
RNA N1-methyladenosine dioxygenase activity
endonuclease activity
exonuclease activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
sulfonate dioxygenase activity
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
metal ion binding
dioxygenase activity
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
Performance Guarantee
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