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Active Motif
ALKBH5 Recombinant Rabbit Monoclonal Antibody, AbFlex®
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Product Details
91441ACTMOTIF
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
CHO cells
Class
Recombinant Monoclonal
Type
Antibody
Immunogen
This ALKBH5 antibody was raised against a peptide of human ALKBH5.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
140mM HEPES/32mM sodium acetate, pH 7.5, with 30% glycerol
Contains
0.035% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
ALKBH5 (RNA demehtylase ALKBH5) is a dioxygenase that demethylates RNA by oxidative demethylation. Specifically, it demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Demethylation of m6A mRNA affects mRNA processing and export. ALKBH5 can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). It requires molecular oxygen, alpha-ketoglutarate and iron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AlkB family member 5, RNA demethylase; alkB, alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; oxoglutarate and iron-dependent oxygenase domain containing; probable alpha-ketoglutarate-dependent dioxygenase ABH5; RNA demethylase ALKBH5; unnamed protein product
Gene Aliases: ABH5; ALKBH5; AW050020; AW215868; E130207K11; OFOXD; OFOXD1; RGD1309496
UniProt ID: (Human) Q6P6C2, (Mouse) Q3TSG4, (Rat) D3ZKD3
Entrez Gene ID: (Human) 54890, (Mouse) 268420, (Rat) 303193
RNA binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
oxidative RNA demethylase activity
metal ion binding
dioxygenase activity
molecular condensate scaffold activity
RNA N6-methyladenosine dioxygenase activity
sulfonate dioxygenase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
poly(A) RNA binding
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
RNA processing factor
response to hypoxia
mRNA processing
regulation of translation
spermatogenesis
regulation of mRNA export from nucleus
cell differentiation
regulation of mRNA stability
gamma-delta T cell proliferation
regulation of mRNA processing
mRNA destabilization
membraneless organelle assembly
positive regulation of defense response to virus by host
mRNA export from nucleus
oxidative single-stranded RNA demethylation
oxidation-reduction process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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