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Invitrogen

ALKBH8 Polyclonal Antibody

View all (6) ALKBH8 antibodies

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ALKBH8 Polyclonal Antibody

Product Details

PA5-96592

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALKBH8 (NP_6201302)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Amount

23 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2808394

Product Specific Information

Immunogen sequence: MDSNHQSNYK LSKTEKKFLR KQIKAKHTLL RHEGIETVSY ATQSLVVANG GLGNGVSRNQ LLPVLEKCGL VDALLMPPNK PYSFARYRTT EESKRAYVTL NGKEVVDDLG QKITLYLNFV EKVQWKELRP QALPPGLMVV EEIISSEEEK MLLESVDWTE DTDNQNSQKS LKHRRVKHFG YEFHYENNNV DKDKPLSGGL PDICESFLEK WLRKGYIKHK PDQMTINQYE; Positive Samples: A-549, NCI-H460, U-87MG, HeLa, K-562; Cellular Location: Cytoplasm, Nucleus

Target Information

Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AlkB homologue 8; alkB, alkylation repair homolog 8; Alkylated DNA repair protein alkB homolog 8; Alpha-ketoglutarate-dependent dioxygenase ALKBH8; probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ALKBH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8; tRNA methyltransferase 9 homolog; tRNA methyltransferase 9 related; unnamed protein product

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Gene Aliases: 4930562C03Rik; 8030431D03Rik; 9430088N01Rik; ABH8; ALKBH8; MRT71; RGD1304687; TRM9; TRMT9; TRMT9A

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UniProt ID: (Human) Q96BT7, (Mouse) Q80Y20

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Entrez Gene ID: (Human) 91801, (Rat) 366783, (Mouse) 67667

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Function(s)
tRNA binding nucleic acid binding RNA binding catalytic activity iron ion binding protein binding methyltransferase activity zinc ion binding S-adenosylmethionine-dependent methyltransferase activity tRNA (uracil) methyltransferase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors transferase activity metal ion binding transition metal ion binding tRNA (carboxymethyluridine(34)-5-O)-methyltransferase activity nucleotide binding rRNA (adenine-N6,N6-)-dimethyltransferase activity sulfonate dioxygenase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity oxidoreductase activity protein C-terminal leucine carboxyl O-methyltransferase activity 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity procollagen-proline dioxygenase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity hypophosphite dioxygenase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity DNA-N1-methyladenine dioxygenase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity C-19 gibberellin 2-beta-dioxygenase activity C-20 gibberellin 2-beta-dioxygenase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity RNA metabolism protein
Process(es)
tRNA wobble uridine modification cellular response to DNA damage stimulus tRNA methylation methylation oxidation-reduction process metabolic process
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