Invitrogen

ALOX12B Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 References

View all (4) ALOX12B antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)
Advanced Verification (1)

ALOX12B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

ALOX12B Antibody (PA5-23608) in IHC (P)

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human tonsil tissue using an ALOX12B polyclonal antibody (Product # PA5-23608), followed by HRP-conjugated secondary antibody and DAB staining.

Product Image Gallery

ALOX12B Antibody in Western Blot (WB) — Western blot analysis using an ALOX12B polyclonal antibody (Product # PA5-23608) in A549 cells lysates (35 µg per lane).

ALOX12B Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of A549 cells using an ALOX12B polyclonal antibody (Product # PA5-23608) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

ALOX12B Antibody — Figure 5. Knockdown of ALOX12B suppresses tumor growth in vivo. (A and B) Western blotting confirmed the knockdown of ALOX12B in vivo. (C) The tumor volume of mice in the shALOX12B group was significantly smaller than that of mice in the NC group. (D) The tumor weight of mice in shALOX12B group was significantly smaller than that of mice in the NC group. *P<0.05. sh, small hairpin; NC, negative control. {KD}

Product Details

PA5-23608

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Flow Cytometry (Flow)

1:10-1:50

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 622-652 amino acids from the C-terminal region of human ALOX12B

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.7 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2541108

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

This gene encodes an enzyme involved in the converstion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ALOX12B promotes carcinogenesis in cervical cancer by regulating the PI3K/ERK1 signaling pathway.

Oncology letters

Jiang T,Zhou B,Li YM,Yang QY,Tu KJ,Li LY

PA5-23608 was used in Western Blotting to show that ALOX12B promotes cell proliferation in cervical cancer via regulation of the PI3K/ERK1 signaling pathway.

Sat Aug 01 00:00:00 EDT 2020

12R-lipoxygenase activity is reduced in photodamaged facial stratum corneum. A novel activity assay indicates a key function in corneocyte maturation.

International journal of cosmetic science

Guneri D,Voegeli R,Munday MR,Lane ME,Rawlings AV

PA5-23608 was used in Flow cytometry to indicate a link between 12R-lipoxygenase activity and CE maturation and stratum corneum integrity.

Sat Jun 01 00:00:00 EDT 2019

Phospho-ΔNp63α regulates AQP3, ALOX12B, CASP14 and CLDN1 expression through transcription and microRNA modulation.

FEBS letters

Ratovitski EA

PA5-23608 was used in western blot to study the role of transcriptional and miRNA mechanisms in the modulation of the expression of AQP3, ALOX12B, CASP14 and CLDN1 by phospho-Deltap63-alpha

Fri Nov 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 12R-lipoxygenase; 12R-LOX; Arachidonate 12-lipoxygenase, 12R-type; Epidermis-type lipoxygenase 12

Gene Aliases: 12R-LOX; ALOX12B; ARCI2

UniProt ID: (Human) O75342

Entrez Gene ID: (Human) 242

Molecular Function: oxygenase

Function(s)

protein lipidation sphingolipid metabolic process positive regulation of gene expression arachidonic acid metabolic process lipoxygenase pathway positive regulation of MAPK cascade linoleic acid metabolic process ceramide biosynthetic process hepoxilin biosynthetic process oxidation-reduction process establishment of skin barrier positive regulation of mucus secretion

Process(es)

arachidonate 12-lipoxygenase activity iron ion binding protein binding oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen linoleate 9S-lipoxygenase activity