Invitrogen

ALOX15 Polyclonal Antibody

View all (20) ALOX15 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

ALOX15 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using an ALOX15 polyclonal antibody (Product # PA5-15065) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

ALOX15 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lymph tissue using an ALOX15 polyclonal antibody (Product # PA5-15065), followed by HRP-conjugated secondary antibody and DAB staining.

ALOX15 Antibody in Western Blot (WB) — Western blot analysis using an ALOX15 polyclonal antibody (Product # PA5-15065) in HeLa (lane 1), A549 (lane 2), K562 (lane 3), MDA-MB435 (lane 4) cell lysates (35 µg per lane).

Product Details

PA5-15065

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunocytochemistry (ICC/IF)

1:10-1:50

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 610-643 amino acids from the C-terminal region of human ALOX15

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Size-exclusion - Dialysis, Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2227014

Target Information

ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 12-LOX; 12/15-lipoxygenase; 15-lipooxygenase-1; 15-LOX; 15-LOX2; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate 15-lipoxygenase; Arachidonate omega-6 lipoxygenase; Hepoxilin A3 synthase Alox15; Linoleate 13S-lipoxygenase; Polyunsaturated fatty acid lipoxygenase ALOX15

Gene Aliases: 12-LOX; 15-LOX-1; 15LOX-1; ALOX15; LOG15

UniProt ID: (Human) P16050

Entrez Gene ID: (Human) 246

Molecular Function: oxygenase

Function(s)

ossification negative regulation of adaptive immune response phosphatidylethanolamine biosynthetic process leukotriene metabolic process inflammatory response positive regulation of cell-substrate adhesion arachidonic acid metabolic process lipoxygenase pathway bone mineralization positive regulation of actin filament polymerization positive regulation of heterotypic cell-cell adhesion response to endoplasmic reticulum stress regulation of peroxisome proliferator activated receptor signaling pathway cellular response to interleukin-13 wound healing apoptotic cell clearance hepoxilin biosynthetic process oxidation-reduction process positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion regulation of engulfment of apoptotic cell lipoxin A4 biosynthetic process

Process(es)

arachidonate 12-lipoxygenase activity iron ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding hepoxilin-epoxide hydrolase activity arachidonate 15-lipoxygenase activity hepoxilin A3 synthase activity eoxin A4 synthase activity