Invitrogen

ALOX15 Polyclonal Antibody

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ALOX15 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HeLa cells using an ALOX15 polyclonal antibody (Product # PA5-15065) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

ALOX15 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lymph tissue using an ALOX15 polyclonal antibody (Product # PA5-15065), followed by HRP-conjugated secondary antibody and DAB staining.

ALOX15 Antibody in Western Blot (WB) — Western blot analysis using an ALOX15 polyclonal antibody (Product # PA5-15065) in HeLa (lane 1), A549 (lane 2), K562 (lane 3), MDA-MB435 (lane 4) cell lysates (35 µg per lane).

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:10-1:50

Immunofluorescence (IF)

1:10-1:50

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Western Blot (WB)

1:1000

Product Specifications

Species

Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 610-643 amino acids from the C-terminal region of human ALOX15

Conjugate

Unconjugated

Form

Liquid

Concentration

2.0 mg/mL

Purification

Size-exclusion - Dialysis, Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2227014

Target Information

ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 12-LOX; 12/15-lipoxygenase; 15-lipooxygenase-1; 15-LOX; 15-LOX2; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate 15-lipoxygenase; Arachidonate omega-6 lipoxygenase

Gene Aliases: 12-LOX; 15-LOX-1; 15LOX-1; ALOX15; LOG15

UniProt ID: (Human) P16050

Entrez Gene ID: (Human) 246

Molecular Function: oxidoreductase oxygenase

Function(s)

ossification negative regulation of adaptive immune response phosphatidylethanolamine biosynthetic process leukotriene metabolic process inflammatory response positive regulation of cell-substrate adhesion arachidonic acid metabolic process lipoxygenase pathway bone mineralization positive regulation of actin filament polymerization positive regulation of heterotypic cell-cell adhesion response to endoplasmic reticulum stress regulation of peroxisome proliferator activated receptor signaling pathway cellular response to interleukin-13 wound healing apoptotic cell clearance hepoxilin biosynthetic process oxidation-reduction process positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion regulation of engulfment of apoptotic cell lipoxin A4 biosynthetic process

Process(es)

arachidonate 12-lipoxygenase activity iron ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding hepoxilin-epoxide hydrolase activity arachidonate 15-lipoxygenase activity hepoxilin A3 synthase activity eoxin A4 synthase activity

ALOX15 Polyclonal Antibody

Product Details

Target Information

References (0)

Bioinformatics

Material safety data sheets (MSDS)

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