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Invitrogen
ALOX5 Polyclonal Antibody
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FIGURE: 1 / 3
ALOX5 Antibody (PA1-46003) in WB
Western Blot was performed using Anti-ALOX5 Polyclonal Antibody (Product # PA1-46003) and a 70 kDa band corresponding to Arachidonate 5-lipoxygenase was observed in THP1 cells treated with both LPS and human TGF beta 1 and U2OS cells treated with Actinomycin D. Expression was also seen in Daudi, Raji and Raw 264.7 as compared to K-562, U-937, MOLT-4 and NIH/3T3 which are reported to be negative for ALOX5. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with Lipopolysaccharide (100 ng/mL) and human TGF beta 1 (1 ng/mL) f... View More
Product Details
PA1-46003
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide made to a C-terminal region within residues 600-674 of human 5-Lipoxygenase.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 30% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: human Peripheral Blood Leukocyte protein.
Target Information
This gene encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5 - Lipoxygenase; 5-lipoxygenase; 5-LO; Arachidonate 5-lipoxygenase; arachidonic acid 5-lipoxygenase; leukotriene A4 synthase; lipoxygenase; LOX-5; lox5; Polyunsaturated fatty acid 5-lipoxygenase; RP11-67C2.3
Gene Aliases: 5-LO; 5-LOX; 5LO; 5LPG; 5LX; AI850497; ALOX5; F730011J02; LOG5; LOX5A
UniProt ID: (Human) P09917, (Rat) P12527, (Mouse) P48999
Entrez Gene ID: (Human) 240, (Rabbit) 100341714, (Bovine) 404074, (Rat) 25290, (Mouse) 11689
arachidonate 5-lipoxygenase activity
arachidonate 12-lipoxygenase activity
iron ion binding
protein binding
oxidoreductase activity
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
hydrolase activity
arachidonate 8(S)-lipoxygenase activity
metal ion binding
dioxygenase activity
oxygenase
long-chain fatty acid metabolic process
negative regulation of endothelial cell proliferation
leukocyte chemotaxis involved in inflammatory response
leukocyte migration involved in inflammatory response
leukotriene production involved in inflammatory response
lipid metabolic process
icosanoid metabolic process
leukotriene metabolic process
response to stress
inflammatory response
humoral immune response
negative regulation of angiogenesis
arachidonic acid metabolic process
leukotriene biosynthetic process
lipoxygenase pathway
positive regulation of bone mineralization
lipid oxidation
dendritic cell migration
glucose homeostasis
long-chain fatty acid biosynthetic process
regulation of fat cell differentiation
regulation of inflammatory response
negative regulation of inflammatory response
regulation of insulin secretion
negative regulation of vascular wound healing
negative regulation of wound healing
regulation of inflammatory response to wounding
regulation of cytokine production involved in inflammatory response
regulation of cellular response to oxidative stress
leukotriene A4 biosynthetic process
regulation of reactive oxygen species biosynthetic process
negative regulation of response to endoplasmic reticulum stress
negative regulation of sprouting angiogenesis
positive regulation of leukocyte adhesion to arterial endothelial cell
lipoxin biosynthetic process
acute inflammatory response
response to nutrient
sensory perception of pain
positive regulation of vasoconstriction
response to hyperoxia
oxidation-reduction process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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