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ALPK1 Antibody (PA5-120710) in WB
Western blot analysis of ALPK1 in extracts of various cell lines. Samples were incubated with ALPK1 Polyclonal antibody (Product # PA5-120710) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-120710
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 340-500 of human ALPK1 (NP_001095876.1).
Conjugate
Form
Liquid
Concentration
1.16 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914282
Product Specific Information
Positive test controls include: HepG2, SW620.
Immunogen sequence: RDDEPVTGKQ ELHSFVKAAF GLTTVHRRLH GETGTVHAAS QLCKEAMGKL YNFSTSSRSQ DREALSQEVM SVIAQVKEHL QVQSFSNVDD RSYVPESFEC RLDKLILHGQ GDFQKILDTY SQHHTSVCEV FESDCGNNKN EQKDAKTGVC ITALKTEIKN I
Target Information
Unlike most eukaryotic kinases, alpha kinases, such as LAK, recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-kinase 1; Alpha-protein kinase 1; Chromosome 4 kinase; lymphocyte alpha-kinase; Lymphocyte alpha-protein kinase
Gene Aliases: 8430410J10Rik; ALPK1; KIAA1527; LAK
UniProt ID: (Human) Q96QP1
Entrez Gene ID: (Human) 80216
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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