Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000-1:4,000 | View 5 publications 5 publications |
Immunohistochemistry (IHC) |
- | View 8 publications 8 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:400 | - |
Immunocytochemistry (ICC/IF) |
Assay-dependent | - |
Flow Cytometry (Flow) |
0.2 µg/1x10^6 cells | - |
ChIP assay (ChIP) |
Assay-dependent | View 1 publication 1 publication |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Published species |
Human, Mouse, Pig, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
ALPL Fusion Protein Ag1664 (23-330 aa encoded by BC021289) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.3 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.1% sodium azide |
Storage conditions |
-20°C |
This antibody can bind the four mentioned alkaline phosphatases.
Immunogen sequence: EKDPKYWRD QAQETLKYAL ELQKLNTNVA KNVIMFLGDG MGVSTVTAAR ILKGQLHHNP GEETRLEMDK FPFVALSKTY NTNAQVPDSA GTATAYLCGV KANEGTVGVS AATERSRCNT TQGNEVTSIL RWAKDAGKSV GIVTTTRVNH ATPSAAYAHS ADRDWYSDNE MPPEALSQGC KDIAYQLMHN IRDIDVIMGG GRKYMYPKNK TDVEYESDEK ARGTRLDGLD LVDTWKSFKP RYKHSHFIWN RTELLTLDPH NVDYLLGLFE PGDMQYELNR NNVTDPSLSE MVVVAIQILR KNPKGFFLL (23-330 aa encoded by BC021289)
Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; tissue-nonspecific ALP
Gene Aliases: ALPL; AP-TNAP; APTNAP; HOPS; TNAP; TNSALP
UniProt ID: (Human) P05186
Entrez Gene ID: (Human) 249
Molecular Function:
phosphatase
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support
