Immunohistochemical analysis of ALS2CR2 in paraffin-embedded human heart tissue using an ALS2CR2 polyclonal antibody (Product # PA1-31834) at a dilution of 3.8 µg/ml. Steamed antigen retrieval was performed using a citrate buffer pH 6, followed by AP-staining.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence CDFPDEKDSYWEF, from C Terminus of the protein sequence according to AAF71042|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||0.2-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31834 detects ALS2CR2 from human samples.
PA1-31834 has been successfully used in Western blot applications.
The PA1-31834 immunogen is: Peptide with sequence CDFPDEKDSYWEF, from C Terminus of the protein sequence according to AAF71042
This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene.
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