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AMH Antibody (PA5-95425) in WB
Western blot analysis of AMH in Lane 1: 293T whole cell lysates, Lane 2: COLO320 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with AMH polyclonal antibody (Product # PA5-95425) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).
Product Details
PA5-95425
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human AMH recombinant protein (Position: A75-E141).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807228
Product Specific Information
Human AMH shares 66.7% amino acid (aa) sequence identity with both mouse and rat AMH.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: 293T whole cell, COLO320 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140 kDa disulfide linked precursor that is cleaved to release the mature 30 kDa homodimer. Originally classified as a fetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AMH; Anti-Muellerian hormone; MIS; Muellerian-inhibiting factor; Muellerian-inhibiting substance; Mullerian inhibiting factor; Mullerian inhibiting substance
Gene Aliases: AMH; MIF; MIS
UniProt ID: (Human) P03971
Entrez Gene ID: (Human) 268
preantral ovarian follicle growth
urogenital system development
Mullerian duct regression
cell-cell signaling
gonadal mesoderm development
sex determination
sex differentiation
aging
positive regulation of gene expression
response to organic cyclic compound
response to drug
positive regulation of NF-kappaB transcription factor activity
negative regulation of ovarian follicle development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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