Invitrogen

AMPD1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (6) AMPD1 antibodies

Datasheet

Datasheet

Product Image Gallery

AMPD1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of AMPD1 in human skeletal muscle and heart muscle tissues using AMPD1 Polyclonal Antibody (Product # PA5-55761). Corresponding AMPD1 RNA-seq data are presented for the same tissues.

AMPD1 Antibody in Western Blot (WB) — Western blot analysis of AMPD1 in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human Skeletal muscle tissue. Samples were probed using an AMPD1 Polyclonal Antibody (Product # PA5-55761).

AMPD1 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of AMPD1 demonstrates antibody specificity. Immunohistochemical analysis of AMPD1 using anti-AMPD1 Polyclonal Antibody (Product # PA5-55761), shows significant staining of AMPD1 in human skeletal muscle and shows minimal or weak staining in human heart muscle tissues. The relative expression levels of AMPD1 within each tissue is shown using RNA-Seq. {RE}

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:200-1:500

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human AMPD1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2637917

Product Specific Information

Immunogen sequence: AEEKQIDDAM RNFAEKVFAS EVKDEGGRQE ISPFDVDEIC PISHHEMQAH IFHLETLSTS TEARRKKRFQ GRKTVNLSIP L

Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 81%.

Target Information

AMP deaminase (AMPD) is an allosteric enzyme involved in the regulation of adenosine metabolism. It catalyzes a central reaction in purine nucleotide biosynthesis where AMP is deaminated to IMP, liberating ammonia. There are three functional isoforms of AMPD. AMPD1 (E.C. No 3.5.4.6.) is the skeletal muscle-specific isoform M located in type II muscle fibers, neuromuscular junctions and in capillaries. This protein is the predominant member of AMPD multi-gene family and is considered as the sensor of the cell's changing energy requirements. AMPD1 deficiency causes irregular muscle metabolism due to lower rate of ATP degradation, phosphocreatine hydrolysis and accumulation of lactic acid. Mutated AMPD1 expression has been detected in neuromuscular disorders, exercise-induced skeletal muscle myopathies and congestive heart failures due to coronary artery diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: adenosine monophosphate deaminase 1 (isoform M); adenosine monophosphate deaminase-1 (muscle); AMP deaminase 1; AMP deaminase isoform M; AMPD; MAD; MADA; Myoadenylate deaminase; RP5-1000E10.1; skeletal muscle AMPD

Gene Aliases: AMPD1; MAD; MADA; MMDD

UniProt ID: (Human) P23109

Entrez Gene ID: (Human) 270

Molecular Function: deaminase hydrolase