Invitrogen

AMPK alpha-1 Polyclonal Antibody

View all (71) AMPK alpha-1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-95517

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human AMPK alpha 1 recombinant protein (Position: F377-R446).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807319

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human SiHa whole cell lysates, human Hela whole cell lysates, human RT4 whole cell lysates, human A549 whole cell lysates, rat brain tissue lysates, rat PC-12 whole cell lysates, mouse brain tissue lysates, mouse NIH/3T3 whole cell lysates.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; activated protein kinase; AMP -activate kinase alpha 1 subunit; AMP activated protein kinase; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha-1 chain; AMPK subunit alpha-1; AMPK subunit pAMPK; AMPK-alpha-2 chain; AMPK2; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; MGC33776; MGC57364; pAMPK; PRKAA; PRKAA2; protein kinase, AMP-activated, alpha 1 catalytic subunit; Tau-protein kinase PRKAA1

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Gene Aliases: AI194361; AI450832; AL024255; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; PRKAA1

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UniProt ID: (Human) Q13131, (Rat) P54645, (Mouse) Q5EG47

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Entrez Gene ID: (Human) 5562, (Rat) 65248, (Mouse) 105787

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Function(s)
chromatin binding protein kinase activity AMP-activated protein kinase activity cAMP-dependent protein kinase activity protein binding ATP binding protein C-terminus binding kinase binding histone serine kinase activity metal ion binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity tau-protein kinase activity [acetyl-CoA carboxylase] kinase activity protein serine/threonine kinase activity eukaryotic elongation factor-2 kinase activator activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
activation of MAPK activity response to hypoxia glucose metabolic process transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation fatty acid biosynthetic process cholesterol biosynthetic process cell cycle arrest signal transduction positive regulation of cell proliferation lipid biosynthetic process response to UV cold acclimation response to gamma radiation positive regulation of autophagy positive regulation of gene expression response to activity Wnt signaling pathway macroautophagy fatty acid oxidation response to caffeine cellular response to nutrient levels negative regulation of TOR signaling regulation of peptidyl-serine phosphorylation histone-serine phosphorylation intracellular signal transduction cellular response to glucose starvation glucose homeostasis regulation of circadian rhythm negative regulation of apoptotic process positive regulation of cholesterol biosynthetic process positive regulation of glycolytic process negative regulation of glucosylceramide biosynthetic process rhythmic process positive regulation of skeletal muscle tissue development negative regulation of lipid catabolic process protein heterooligomerization fatty acid homeostasis regulation of vesicle-mediated transport cellular response to hydrogen peroxide cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to organonitrogen compound cellular response to hypoxia response to camptothecin regulation of signal transduction by p53 class mediator regulation of energy homeostasis negative regulation of glucose import in response to insulin stimulus autophagy negative regulation of translation response to hydrogen peroxide positive regulation of gluconeogenesis positive regulation of fatty acid oxidation positive regulation of glucose import lipid metabolic process fatty acid metabolic process steroid biosynthetic process steroid metabolic process cholesterol metabolic process sterol biosynthetic process phosphorylation chromatin modification
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