Proteintech

AMPK alpha 2 Polyclonal Antibody

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AMPK alpha 2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using AMPK Alpha 2 antibody (Product # 18167-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

AMPK alpha 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

AMPK alpha 2 Antibody in Western Blot (WB) — Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

AMPK alpha 2 Antibody in Immunoprecipitation (IP) — IP result of anti-AMPK alpha 2 (IP:18167-1-AP, 4ug; Detection:18167-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3600ug.

Product Details

18167-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 38 publications 38 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AMPK alpha 2 Fusion Protein Ag12796 (215-552 aa encoded by BC069680)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: DDEHVPTLF KKIRGGVFYI PEYLNRSVAT LLMHMLQVDP LKRATIKDIR EHEWFKQDLP SYLFPEDPSY DANVIDDEAV KEVCEKFECT ESEVMNSLYS GDPQDQLAVA YHLIIDNRRI MNQASEFYLA SSPPSGSFMD DSAMHIPPGL KPHPERMPPL IADSPKARCP LDALNTTKPK SLAVKKAKWH LGIRSQSKPY DIMAEVYRAM KQLDFEWKVV NAYHLRVRRK NPVTGNYVKM SLQLYLVDNR SYLLDFKSID DEVVEQRSGS STPQRSCSAA GLHRPRSSFD STTAESHSLS GSLTGSLTGS TLSSVSPRLG SHTMDFFEMC ASLITTLAR (215-552 aa encoded by BC069680)

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mailuoning oral liquid attenuates convalescent cerebral ischemia by inhibiting AMPK/mTOR-associated apoptosis and promoting CREB/BDNF-mediated neuroprotection.

Journal of ethnopharmacology

Liu X,Fan L,Li J,Bai Z,Wang Y,Liu Y,Jiang H,Tao A,Li X,Zhang H,Tan N

Tue Dec 05 00:00:00 EST 2023

Astaxanthin promotes mitochondrial biogenesis and antioxidant capacity in chronic high-intensity interval training.

European journal of nutrition

Wang Y,Chen X,Baker JS,Davison GW,Xu S,Zhou Y,Bao X

Sat Apr 01 00:00:00 EDT 2023

Quantification and Proteomic Characterization of β-Hydroxybutyrylation Modification in the Hearts of AMPKα2 Knockout Mice.

Molecular & cellular proteomics : MCP

Ding WJ,Li XH,Tang CM,Yang XC,Sun Y,Song YP,Ling MY,Yan R,Gao HQ,Zhang WH,Yu N,Feng JC,Zhang Z,Xing YQ

Wed Feb 01 00:00:00 EST 2023

Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function.

Animal nutrition (Zhongguo xu mu shou yi xue hui)

He L,Zhou X,Wu Z,Feng Y,Liu D,Li T,Yin Y

Thu Sep 01 00:00:00 EDT 2022

Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion.

Nature

Wang JQ,Li LL,Hu A,Deng G,Wei J,Li YF,Liu YB,Lu XY,Qiu ZP,Shi XJ,Zhao X,Luo J,Song BL

Mon Aug 01 00:00:00 EDT 2022

Phosphorylated STYK1 restrains the inhibitory role of EGFR in autophagy initiation and EGFR-TKIs sensitivity.

Cell insight

Zhou C,Dong X,Wang M,Qian X,Hu M,Liang K,Liang Y,Zhang R,Huang Y,Lyu H,Xiao S,Tang Y,Ali DW,Michalak M,Chen XZ,Tang J

Mon Aug 01 00:00:00 EDT 2022

Phosphorylated STYK1 restrains the inhibitory role of EGFR in autophagy initiation and EGFR-TKIs sensitivity.

Cell insight

Zhou C,Dong X,Wang M,Qian X,Hu M,Liang K,Liang Y,Zhang R,Huang Y,Lyu H,Xiao S,Tang Y,Ali DW,Michalak M,Chen XZ,Tang J

Mon Aug 01 00:00:00 EDT 2022

Metformin Relieves Bortezomib-Induced Neuropathic Pain by Regulating AMPKa2-Mediated Autophagy in the Spinal Dorsal Horn.

Neurochemical research

Liu M,Zhao YT,Lv YY,Xu T,Li D,Xiong YC,Xin WJ,Lin SY

Fri Jul 01 00:00:00 EDT 2022

Metformin Relieves Bortezomib-Induced Neuropathic Pain by Regulating AMPKa2-Mediated Autophagy in the Spinal Dorsal Horn.

Neurochemical research

Liu M,Zhao YT,Lv YY,Xu T,Li D,Xiong YC,Xin WJ,Lin SY

Fri Jul 01 00:00:00 EDT 2022

Targeting USP9X-AMPK Axis in ARID1A-Deficient Hepatocellular Carcinoma.