Proteintech

AMPK alpha 2 Polyclonal Antibody

Product Image Gallery

AMPK alpha 2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of MCF-7 cells, using PRKAA2 antibody 18167-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

AMPK alpha 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

AMPK alpha 2 Antibody in Western Blot (WB) — Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

AMPK alpha 2 Antibody in Immunoprecipitation (IP) — IP result of anti-AMPK alpha 2 (IP:18167-1-AP, 4ug; Detection:18167-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3600ug.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2000

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AMPK alpha 2 Fusion Protein Ag12796

Conjugate

Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP-activated protein kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMPK alpha 2; AMPK alpha-2 chain; AMPK subunit alpha-2; AMPK-alpha-2 chain; EC 2.7.11.1; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; prkaa2; protein kinase, AMP-activated, alpha 2 catalytic subunit

Gene Aliases: 2310008I11Rik; A830082D05; AMPK; AMPK2; AMPKa2; AMPKalpha2; PRKAA; PRKAA2

UniProt ID: (Human) P54646, (Rat) Q09137, (Mouse) Q8BRK8

Entrez Gene ID: (Human) 5563, (Rat) 78975, (Mouse) 108079

Molecular Function: kinase non-receptor serine/threonine protein kinase protein kinase transferase