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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • ANKIB1 Antibodies

          Bethyl Laboratories

          ANKIB1 Polyclonal Antibody

          View all (3) ANKIB1 antibodies

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          Cite ANKIB1 Polyclonal Antibody

          • Antibody Testing Data (1)
          ANKIB1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          ANKIB1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          ANKIB1 Antibody (A301-368A) in WB

          Detection of human ANKIB1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ANKIB1 antibody (Product # A301-368A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. ANKIB1 was also immunoprecipitated by rabbit anti-ANKIB1 antibody A301-367A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 second... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ANKIB1 Antibody in Western Blot (WB)
          ANKIB1 Polyclonal Antibody

          Product Details

          A301-368A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          -

          Immunoprecipitation (IP)

          2-5 µg/mg lysate
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 1039 and 1089 of human ankyrin repeat and IBR domain-containing protein 1.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.20 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          TBS, pH 7.0 to 8.0, with 0.1% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Target Information

          Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Ankyrin repeat and IBR domain-containing protein 1

          View more View less

          Gene Aliases: ANKIB1; KIAA1386

          View more View less

          UniProt ID: (Human) Q9P2G1

          View more View less

          Entrez Gene ID: (Human) 54467

          View more View less

          Function(s)
          protein binding zinc ion binding ubiquitin conjugating enzyme binding ubiquitin protein ligase activity ubiquitin-protein ligase
          Process(es)
          protein polyubiquitination positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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