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Immunofluorescent analysis of ANKRD32 showing staining in the nucleus of U87-MG cells. U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an ANKRD32 polyclonal antibody (Product # PA5-31991) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 627 and 924 of Human ANKRD32|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31991 targets ANKRD32 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-31991 immunogen is recombinant fragment corresponding to a region within amino acids 627 and 924 of Human ANKRD32.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ankyrin repeat domain 32; Ankyrin repeat domain-containing protein 32; BRCT domain containing 1; BRCT domain-containing protein 1; BRCTD1; BRCTx; SMC5-SMC6 complex localization factor protein 1; Smc5/6 localization factor 1
ANKRD32; BRCTD1; BRCTx; SLF1