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Invitrogen
AP1M1 Polyclonal Antibody
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FIGURE: 1 / 5
AP1M1 Antibody (PA5-104319) in ICC/IF
Immunofluorescent analysis of AP1M1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and AP1M1 polyclonal antibody (Product # PA5-104319) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-104319
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human AP1M1(Accession Q9BXS5), corresponding to amino acid residues I291-I341.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total AP1M1.
Target Information
The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adapter-related protein complex 1 subunit mu-1; adaptor protein complex AP-1 mu-1 subunit; Adaptor protein complex AP-1 subunit mu-1; adaptor related protein complex 1 mu 1 subunit; adaptor-related protein complex 1 mu-1 subunit; Adaptor-related protein complex 1 subunit mu-1; adaptor-related protein complex AP-1, mu subunit 1; AP-1 complex subunit mu-1; AP-1 mu1; AP-mu chain family member mu1A; ap1m1 {ECO:0000312|RGD:1307653}; clathrin adaptor protein AP47; clathrin assembly protein complex 1 medium chain 1; Clathrin assembly protein complex 1 mu-1 medium chain 1; clathrin assembly protein complex 1, medium chain; clathrin assembly protein complex AP1, mu subunit; Clathrin coat assembly protein AP47; Clathrin coat-associated protein AP47; golgi adaptor AP-1 47 kDa protein; Golgi adaptor HA1/AP1 adaptin mu-1 subunit; HA1 47 kDa subunit; Mu-adaptin 1; Mu1A-adaptin; unnamed protein product
Gene Aliases: [m]1A; AA408894; Adtm1A; AP1M1; AP47; CLAPM2; CLTNM; MU-1A; mu1A
UniProt ID: (Human) Q9BXS5, (Rat) Q32Q06, (Mouse) P35585
Entrez Gene ID: (Human) 8907, (Rat) 306332, (Mouse) 11767
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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