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Invitrogen
AP1M1 Polyclonal Antibody
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FIGURE: 1 / 1
AP1M1 Antibody (PA5-87656) in WB
Western blot analysis of AP1M1 in various lysates. Samples were incubated with AP1M1 Polyclonal antibody (Product # PA5-87656) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-87656
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human AP1M1 (NP_1158821)
Conjugate
Form
Liquid
Concentration
0.49 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MSASAVYVLD LKGKVLICRN YRGDVDMSEV EHFMPILMEK EEEGMLSPIL AHGGVRFMWI KHNNLYLVAT SKKNACVSLV FSFLYKVVQV FSEYFKELEE ESIRDNFVII YELLDELMDF GYPQTTDSKI LQEYITQEGH KLETGAPRPP ATVTNAVSWR; Positive Samples: HeLa, Jurkat, HepG2, HL-60, HT-1080, Mouse brain, Mouse kidney, Mouse liver, Rat testis; Cellular Location: Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus, Peripheral membrane protein, clathrin-coated vesicle membrane
Target Information
The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adapter-related protein complex 1 subunit mu-1; adaptor protein complex AP-1 mu-1 subunit; Adaptor protein complex AP-1 subunit mu-1; adaptor-related protein complex 1 mu 1 subunit; adaptor-related protein complex 1 mu-1 subunit; Adaptor-related protein complex 1 subunit mu-1; adaptor-related protein complex 1, mu 1 subunit; adaptor-related protein complex AP-1, mu subunit 1; AP-1 complex subunit mu-1; AP-mu chain family member mu1A; ap1m1 {ECO:0000312|RGD:1307653}; clathrin adaptor protein AP47; clathrin assembly protein complex 1 medium chain 1; Clathrin assembly protein complex 1 mu-1 medium chain 1; clathrin assembly protein complex 1, medium chain; clathrin assembly protein complex AP1, mu subunit; Clathrin coat assembly protein AP47; Clathrin coat-associated protein AP47; golgi adaptor AP-1 47 kDa protein; Golgi adaptor HA1/AP1 adaptin mu-1 subunit; HA1 47 kDa subunit; Mu-adaptin 1; Mu1A-adaptin
Gene Aliases: [m]1A; AA408894; Adtm1A; AP1M1; AP47; CLAPM2; CLTNM; MU-1A; mu1A
UniProt ID: (Human) Q9BXS5, (Rat) Q32Q06, (Mouse) P35585
Entrez Gene ID: (Human) 8907, (Rat) 306332, (Mouse) 11767
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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