|Tested species reactivity||Human, Mouse, Sheep, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues S(420) H T D N N A K S S D K E E K H R K(437) of human AP2 alpha.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25327 detects AP2 alpha from human samples. PA1-25327 is expected to cross react with mouse (94% conserved), rat (94% conserved), chicken (88% conserved), and ovine (94% conserved) due to sequence homology.
PA1-25327 has been successfully used in Western blot and immunofluorescence procedures.
The PA1-25327 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues S(420) H T D N N A K S S D K E E K H R K(437) of human AP2 alpha.
The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.