Invitrogen

AP2 alpha Polyclonal Antibody

View all (14) AP2 alpha antibodies

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Questions & Answers

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AP2 alpha Antibody in Western Blot (WB) — Western blot using AP2 alpha Polyclonal Antibody (Product # PA1-31776) shows detection of a band just below 100 kDa correspond-ing to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 µg/mL of the immunizing peptide which effect-ively blocks the specific reactivity of this antibody with AP2A. Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche) using a 60-sec exposure time.

Product Details

PA1-31776

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
ELISA (ELISA)	1:5,000-1:20,000	-
Immunoprecipitation (IP)	1:100	-

Product Specifications
Species Reactivity	Dog, Chicken, Chimpanzee, Human, Mouse, Non-human primate, Rat
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	synthetic peptide AVSKGDGMRGLAC, corresponding to amino acids 3 - 14 of Human AP2 alpha. View antigen
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains	0.01% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2287244

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. TFAP2A is the only AP-2 protein required for early morphogenesis of the lens vesicle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: activating enhancer-binding protein 2 alpha; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; AP-2 transcription factor; AP2-alpha; FLJ51761; OTTHUMP00000214240; OTTHUMP00000214243; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha; transcription factor AP-2-alpha-like; transcription factor AP2

Gene Aliases: AP-2; Ap-2 (a); AP-2alpha; Ap2; AP2alpha; AP2TF; BOFS; Tcfap2a; TFAP2; TFAP2A

UniProt ID: (Human) P05549, (Mouse) P34056

Entrez Gene ID: (Dog) 488213, (Human) 7020, (Chicken) 395982, (Mouse) 21418, (Rat) 306862

Molecular Function: general transcription factor

negative regulation of transcription from RNA polymerase II promoter kidney development optic vesicle morphogenesis optic cup structural organization transcription from RNA polymerase II promoter sensory perception of sound negative regulation of cell proliferation response to water deprivation positive regulation of gene expression retina layer formation negative regulation of transcription by competitive promoter binding Schwann cell development trigeminal nerve development oculomotor nerve formation positive regulation of bone mineralization response to lipopolysaccharide embryonic forelimb morphogenesis inner ear morphogenesis negative regulation of apoptotic process positive regulation of neuron apoptotic process regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic cranial skeleton morphogenesis palate development bone morphogenesis eyelid development in camera-type eye positive regulation of tooth mineralization cellular response to iron ion negative regulation of reactive oxygen species metabolic process neural tube closure lens morphogenesis in camera-type eye outflow tract morphogenesis keratinocyte development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter embryonic pattern specification embryonic body morphogenesis neural crest cell development anterior neuropore closure forebrain neuron development positive regulation of cell migration forelimb morphogenesis negative regulation of epidermal growth factor receptor signaling pathway negative regulation of neuron apoptotic process skin development regulation of neuron differentiation sympathetic nervous system development embryonic camera-type eye morphogenesis skeletal system morphogenesis epidermis morphogenesis lens induction in camera-type eye face morphogenesis cornea development in camera-type eye basement membrane organization response to organic substance regulation of cell proliferation metanephric nephron development

Process(es)

transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription coactivator activity protein binding transcription factor binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding protein dimerization activity RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription coactivator activity RNA polymerase II transcription corepressor activity transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding