Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:3,000 | - |
Immunoprecipitation (IP) |
1:100-1:500 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fragment corresponding to a region within amino acids 236 and 437 of Human AP2 alpha |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.64 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
0.1M tris glycine, pH 7, with 20% glycerol |
Contains |
0.01% thimerosal |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2547243 |
Recommended positive controls: HeLa, C8D30, Rat2.
Predicted reactivity: Mouse (98%), Rat (99%), Zebrafish (87%), Xenopus laevis (93%), Chicken (98%), Sheep (99%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. TFAP2A is the only AP-2 protein required for early morphogenesis of the lens vesicle.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: activating enhancer-binding protein 2 alpha; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; AP-2 transcription factor; AP2-alpha; FLJ51761; OTTHUMP00000214240; OTTHUMP00000214243; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha
Gene Aliases: AP-2; Ap-2 (a); AP-2alpha; Ap2; AP2alpha; AP2TF; BOFS; Tcfap2a; TFAP2; TFAP2A
UniProt ID: (Human) P05549, (Mouse) P34056
Entrez Gene ID: (Human) 7020, (Mouse) 21418, (Rat) 306862
Molecular Function:
general transcription factor
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