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AP2A2 Antibody (PA5-112102) in WB
Western Blot analysis of AP2A2 using AP2A2 Polyclonal Antibody (Product # PA5-112102) at a dilution of 1:500. Lane A: HepG2 whole cell lysate, Lane B: HeLa whole cell lysate, Lane C: Jurkat whole cell lysate, Lane D: A431 whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG H&L (Dylight 800) at a 1:10,000 dilution. Developed using the Odyssey technique and performed under reducing conditions Predicted band size: 104 kDa. Observed band size: 120 kDa.
Product Details
PA5-112102
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human Ap2a2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2866838
Product Specific Information
This antibody reacts with: Human, Mouse, Rat (Species predicted to react based on 100% sequence homology).
Target Information
Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Alpha adaptin is a subunit of the plasma membrane adaptor. It binds polyphosphoinositides.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 100 kDa coated vesicle protein C; adapter-related protein complex 2 subunit alpha-2; adaptin alpha A; Adaptin alpha B; adaptin, alpha B; Adaptor protein complex AP-2 subunit alpha-2; adaptor related protein complex 2, alpha 2 subunit; Adaptor-related protein complex 2 subunit alpha-2; adaptor-related protein complex 2, alpha 2 subunit; ADTAA; Alpha adaptin A; Alpha adaptin C; Alpha-adaptin C; alpha-adaptin C; Huntingtin interacting protein J; Alpha1 adaptin; Alpha2 adaptin; Alpha2-adaptin; AP-2 complex subunit alpha-2; AP2 alpha; AP2A1; CLAPA1; Clathrin assembly; Clathrin assembly protein complex 2 alpha-C large chain; clathrin-associated/assembly/adaptor protein, large, alpha 2; HIP 9; HIP-9; Huntingtin yeast partner J; Huntingtin-interacting protein 9; Huntingtin-interacting protein J; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
Gene Aliases: ADTAB; AP2A2; CLAPA2; HIP-9; HIP9; HYPJ; KIAA0899
UniProt ID: (Human) O94973
Entrez Gene ID: (Human) 161
intracellular protein transport
microtubule-based movement
antigen processing and presentation of exogenous peptide antigen via MHC class II
negative regulation of epidermal growth factor receptor signaling pathway
ephrin receptor signaling pathway
regulation of defense response to virus by virus
Wnt signaling pathway, planar cell polarity pathway
clathrin-mediated endocytosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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