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Invitrogen
AP2M1 Polyclonal Antibody
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FIGURE: 1 / 1
AP2M1 Antibody (PA5-96136) in WB
Western blot analysis of AP2M1 in extracts of various cell lines. Samples were incubated with AP2M1 Polyclonal antibody (Product # PA5-96136) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-96136
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 134-433 of human AP2M1 (NP_0010203761)
Conjugate
Form
Liquid
Concentration
0.34 mg/mL
Amount
34 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: TQQGIKSQTK EEQSQITSQV TGQIGWRREG IKYRRNELFL DVLESVNLLM SPQGQVLSAH VSGRVVMKSY LSGMPECKFG MNDKIVIEKQ GKGTADETSK SGKQSIAIDD CTFHQCVRLS KFDSERSISF IPPDGEFELM RYRTTKDIIL PFRVIPLVRE VGRTKLEVKV VIKSNFKPSL LAQKIEVRIP TPLNTSGVQV ICMKGKAKYK ASENAIVWKI KRMAGMKESQ ISAEIELLPT NDKKKWARPP ISMNFEVPFA PSGLKVRYLK VFEPKLNYSD HDVIKWVRYI GRSGIYETRC; Positive Samples: HeLa, 293T, MCF7, A-549, U-87MG; Cellular Location: Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit
Target Information
The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adaptin-mu2; Adaptor protein complex AP-2 subunit mu; adaptor related protein complex 2 mu 1 subunit; Adaptor-related protein complex 2 subunit mu; AP-2 complex subunit mu; AP-2 mu 2 chain; AP-2 mu chain; clathrin adaptor complex AP2, mu subunit; clathrin assembly protein complex 2 medium chain; Clathrin assembly protein complex 2 mu medium chain; clathrin coat adaptor protein AP50; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; clathrin-associated/assembly/adaptor protein, medium 1; HA2 50 kDA subunit; KIAA0109; Plasma membrane adaptor AP-2 50 kDa protein; plasma membrane adaptor AP-2 50kDA protein; unnamed protein product
Gene Aliases: AP2M1; AP50; CLAPM1; KIAA0109; MRD60; mu2
UniProt ID: (Human) Q96CW1
Entrez Gene ID: (Human) 1173
positive regulation of receptor internalization
intracellular protein transport
endocytosis
membrane budding
protein transport
vesicle-mediated transport
receptor internalization
synaptic vesicle endocytosis
protein-containing complex assembly
clathrin-mediated endocytosis
regulation of vesicle size
postsynaptic neurotransmitter receptor internalization
vesicle-mediated transport in synapse
positive regulation of synaptic vesicle endocytosis
negative regulation of protein localization to plasma membrane
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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