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Bethyl Laboratories
AP4 Polyclonal Antibody
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FIGURE: 1 / 1
AP4 Antibody (A302-761A) in WB
Detection of human AP4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-AP4 antibody (Product # A302-761A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 3 µg/mg lysate. AP4 was also immunoprecipitated by rabbit anti-AP4 antibody A302-760A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Product Details
A302-761A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 288 and 338 of human Activating Enhancer-Binding Protein 4.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Transcription factors of the basic helix-loop-helix-zipper(bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating enhancer-binding protein 4; basic helix-loop-helix DNA-binding protein; bHLHc41; Class C basic helix-loop-helix protein 41; TFAP4; Transcription factor AP-4; transcription factor AP-4 (activating enhancer binding protein 4)
Gene Aliases: AP-4; BHLHC41; TFAP4
UniProt ID: (Human) Q01664
Entrez Gene ID: (Human) 7023
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
protein binding
protein homodimerization activity
histone deacetylase binding
sequence-specific DNA binding
protein dimerization activity
E-box binding
sequence-specific double-stranded DNA binding
regulation of transcription from RNA polymerase II promoter
negative regulation of cell proliferation
negative regulation of gene expression
DNA damage response, signal transduction by p53 class mediator
positive regulation of apoptotic process
negative regulation of DNA binding
negative regulation by host of viral transcription
positive regulation by host of viral transcription
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein-containing complex assembly
cellular response to dexamethasone stimulus
regulation of mitotic cell cycle phase transition
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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