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Invitrogen
APAF1 Polyclonal Antibody
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FIGURE: 1 / 3
APAF1 Antibody (PA5-85121) in IHC (P)
APAF1 Polyclonal Antibody detects APAF1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. APAF1 Polyclonal Antibody (Product # PA5-85121) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Product Details
PA5-85121
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human APAF1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.51 mg/mL
Amount
51 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (85%), Rat (85%), Bovine (90%).
Positive Control: Target recombinant protein.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: APAF-1; Apaf-1XL; Apaf1 variant; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; cytochrome c and dATP-dependent procaspase-9 activation factor; DKFZp781B1145; forebrain overgrowth; KIAA0413; similar to C. elegans cell death gene ced-4
Gene Aliases: 6230400I06Rik; APAF-1; APAF1; Apaf1l; CED4; fog; KIAA0413; mKIAA0413
UniProt ID: (Human) O14727, (Rat) Q9EPV5, (Mouse) O88879
Entrez Gene ID: (Human) 317, (Rat) 78963, (Mouse) 11783
response to hypoxia
neural tube closure
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
multicellular organism development
brain development
aging
response to nutrient
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
cardiac muscle cell apoptotic process
cell differentiation
forebrain development
glial cell apoptotic process
protein homooligomerization
neuron apoptotic process
cellular response to transforming growth factor beta stimulus
apoptotic signaling pathway
regulation of apoptotic DNA fragmentation
positive regulation of apoptotic signaling pathway
regulation of apoptotic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
kidney development
nervous system development
positive regulation of apoptotic process
system development
response to G1 DNA damage checkpoint signaling
intrinsic apoptotic signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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