|Western Blot (WB)||4 µg/ml|
|Immunohistochemistry (IHC)||See 2 publications below|
|Species reactivity||Mouse, Rat|
|Published species||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) N H L E G S A E V E V A D E A P(17) C of rat Mint1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-070 detects munc-18 interacting protein 1 (Mint1) from rat and mouse brain extract as well as extract from HEK293 cells overexpressing the rat gene.
PA1-070 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~130 kDa protein representing Mint1 from rat forebrain postnuclear supernatant.
The PA1-070 immunogen is a synthetic peptide corresponding to residues M(1) N H L E G S A E V E V A D E A P(17) C of rat Mint1. This peptide (Cat. # PEP-157) is available for use in neutralization and control experiments.
The munc-18 interacting protein (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three Mint isoforms, Mint1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint1 and 2 appear to be expressed exclusively in the brain and are found to bind to Munc18, an essential component of the synaptic vesicle fusion machinery. Mint3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that mint3 does not interact with munc-18. Mint3 has been found to interact with the Alzheimer's Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that mint3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Adapter protein X11alpha; amyloid beta (A4) precursor protein-binding family A APBA1: amyloid beta (A4) precursor protein-binding family A member 1 (X11); amyloid beta (A4) precursor protein-binding, family A, APBA1: amyloid beta (A4) precursor protein-binding, family A, member 1 (X11); Amyloid beta A4 precursor protein-binding family A member 1; Mint-1; Munc-18 Interacting Protein 1; Neuron-specific X11 protein; Neuronal Munc18-1-interacting; Neuronal Munc18-1-interacting protein 1; UROP11-110
Gene Aliases: 6430513E09Rik; Apba1; Lin-10; Mint; Mint1; Urop11; X11; X11alpha
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.