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Bethyl Laboratories
APC1 Polyclonal Antibody
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FIGURE: 1 / 1
APC1 Antibody (A301-653A) in WB
Detection of human and mouse APC1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-APC1 antibody (Product # A301-653A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. APC1 was also immunoprecipitated by rabbit anti-APC1 antibody BL4615, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure t... View More
Product Details
A301-653A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 500 and 550 of human anaphase promoting complex subunit 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC1, also known as mitotic checkpoint regulator (MCPR), is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anaphase-promoting complex 1 (meiotic checkpoint regulator); Anaphase-promoting complex subunit 1; APC1; Cyclosome subunit 1; meiotic check point regulator; Mitotic checkpoint regulator; Testis-specific gene 24 protein
Gene Aliases: 2610021O03Rik; AI047775; AI853536; ANAPC1; APC1; AW547281; MCPR; TSG24
UniProt ID: (Human) Q9H1A4, (Mouse) P53995
Entrez Gene ID: (Human) 64682, (Mouse) 17222
mitotic nuclear division
anaphase-promoting complex-dependent catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
protein K11-linked ubiquitination
cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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