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APC10 Polyclonal Antibody
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FIGURE: 1 / 3
APC10 Antibody (600-401-Y31) in ICC/IF
Immunocytochemistry of APC10 antibody. Tissue: K562 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: APC10 antibody at 5 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: APC10 is cytosolic. Staining: APC10 as precipitated purple signal with blue nuclear counterstain.
Product Details
600-401-Y31
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-APC10 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide near the internal region of human APC10.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC10 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. It has been suggested that APC10 plays a role to regulate the binding of specific substrates to the APC/C complex, similar to that of coactivators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Anaphase-promoting complex subunit 10; APC10; Cyclosome subunit 10; DKFZp564L0562
Gene Aliases: 1500026N15Rik; A830003M23Rik; ANAPC10; APC10; DOC1
UniProt ID: (Human) Q9UM13, (Mouse) Q8K2H6
Entrez Gene ID: (Human) 10393, (Mouse) 68999, (Rat) 361389
regulation of mitotic cell cycle
protein ubiquitination
anaphase-promoting complex-dependent catabolic process
cell division
regulation of meiotic cell cycle
protein K48-linked ubiquitination
protein K11-linked ubiquitination
protein branched polyubiquitination
cell cycle
mitotic nuclear division
regulation of mitotic metaphase/anaphase transition
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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