Western blot detection of APC10 in 20µg of HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2). Samples were loaded onto a 10% SDS-PAGE gel and probed using an APC10 polyclonal antibody (Product # PA1-31765) at a dilution of 1:500. Primary antibody was reacted with the membrane at room temperature for 1 hr. After subsequent washing, a 1:2000 dilution of HRP conjugated Goat-anti-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide GADPKQLERTG, corresponding to amino acids 10-20 of Human Apc10.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31765 detects APC10 from human samples.
PA1-31765 has been successfully used in Western blot applications.
The PA1-31765 immunogen is: Synthetic peptide: GADPKQLERTG, corresponding to amino acids 10-20 of Human Apc10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.