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APC3 Polyclonal Antibody
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FIGURE: 1 / 2
APC3 Antibody (600-401-Y33) in IHC (P)
Immunohistochemistry of APC3 antibody. Tissue: Rat liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: APC3 antibody at 5 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: APC3 is nuclear. Staining: APC3 as precipitated purple signal with blue nuclear counterstain.
Product Details
600-401-Y33
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-APC3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid synthetic peptide near the C-terminus of human APC3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AN CDC27Hs; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC3; CDC27 homolog; CDC27Hs; cell division cycle 27 homolog; Cell division cycle protein 27 homolog; D0S1430E, D17S978E; H-NUC; nuc2 homolog; nuclear DNA binding protein; unnamed protein product
Gene Aliases: AI452358; ANAPC3; APC3; BC023187; CDC27; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2
UniProt ID: (Human) G3V1C4, (Mouse) A2A6Q5, (Rat) Q4V8A2
Entrez Gene ID: (Human) 996, (Mouse) 217232, (Rat) 360643
metaphase/anaphase transition of mitotic cell cycle
regulation of mitotic cell cycle
protein ubiquitination
anaphase-promoting complex-dependent catabolic process
neuron projection development
cell division
regulation of meiotic cell cycle
protein K48-linked ubiquitination
protein K11-linked ubiquitination
protein branched polyubiquitination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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