|Tested species reactivity||Human, Mouse, Non-human primate, Rabbit, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Affinity purified human Apurinic/Apyrimidinic Endonuclease (APE/ref-1) fusion protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:10-1:500|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||7 µg/ml|
|Neutralization (Neu)||50-200 molar excess|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In IHC applications, this antibody can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein.
This antibody can be used on frozen sections, fixed-paraffin sections and cytospin preps.
Suggested positive control: Hela whole cell extract, antigen standard for APEX1 (transient overexpression lysate).
The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme has been shown to function as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1a as well as other transcription factors. Recently, APE has also been shown to control p53 activity through redox alteration. APE is also linked to apoptosis, associated with thioredoxin, and altered levels of APE have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.