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Invitrogen
APE1 Polyclonal Antibody
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FIGURE: 1 / 5
APE1 Antibody (PA5-80342) in ICC/IF
Immunofluorescence staining of APE1 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with APE1 Polyclonal Antibody (Product # PA5-80342, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Product Details
PA5-80342
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human APEX1/AP/APEx/Ref-1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human APEX1/AP/APEx/Ref-1.
Target Information
Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AP endonuclease 1; AP endonuclease class I; AP lyase; APEN; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; Apurinic-apyrimidinic endonuclease 1; apurinic/a; Apurinic/apy; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic); DNA repair nuclease/redox regulator APEX1; DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; HAP1; protein REF-1; redox factor; redox factor 1; Redox factor-1; REF-1; unnamed protein product
Gene Aliases: APE; APE1; APEN; APEX; APEX1; APX; HAP1; REF1
UniProt ID: (Human) P27695
Entrez Gene ID: (Human) 328
DNA binding
damaged DNA binding
double-stranded telomeric DNA binding
transcription coactivator activity
transcription corepressor activity
RNA binding
catalytic activity
DNA-(apurinic or apyrimidinic site) lyase activity
nuclease activity
endonuclease activity
endodeoxyribonuclease activity
RNA-DNA hybrid ribonuclease activity
exonuclease activity
phosphodiesterase I activity
uracil DNA N-glycosylase activity
protein binding
phosphoric diester hydrolase activity
3'-5'-exodeoxyribonuclease activity
double-stranded DNA exodeoxyribonuclease activity
double-stranded DNA 3'-5' exodeoxyribonuclease activity
3'-5' exonuclease activity
oxidoreductase activity
hydrolase activity
chromatin DNA binding
deoxyribonuclease (pyrimidine dimer) activity
metal ion binding
class II DNA-(apurinic or apyrimidinic site) endonuclease activity
phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands
DNA-(abasic site) binding
telomere maintenance
DNA repair
base-excision repair
base-excision repair, gap-filling
DNA catabolic process
DNA recombination
cellular response to DNA damage stimulus
regulation of apoptotic process
regulation of mRNA stability
hypomethylation of CpG island
cell redox homeostasis
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
telomere maintenance via base-excision repair
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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