|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 13 amino acid peptide from near the amino terminus of human APH1a.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Despite its predicted molecular weight, APH1 often migrates at aberrant locations in SDS-PAGE. A suggested positive control is RAW264.7 cell lysate.
PA5-20317 can be used with blocking peptide PEP-0437.
APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the -gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the -gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer and quote;s disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the -gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.