Invitrogen

APH1 Polyclonal Antibody

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APH1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of human brain tissue using a APH1 polyclonal antibody (Product # PA5-20318) at a 5 µg/mL dilution.

APH1 Antibody in Western Blot (WB) — Western blot analysis of human brain tissue lysate using a APH1 polyclonal antibody (Product # PA5-20318) at (A) 0.5 and (B) 1 µg/mL.

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

5 µg/mL

Western Blot (WB)

0.5-1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

An 18 amino acid peptide from near the center of human APH1.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

RRID

AB_11152148

Product Specific Information

Despite its predicted molecular weight, APH1 often migrates at aberrant locations in SDS-PAGE. A suggested positive control is human brain tissue lysate.

PA5-20318 can be used with blocking peptide PEP-0438.

Target Information

APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the -gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the -gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer's disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the -gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: anterior pharynx defective 1 homolog A; anterior pharynx defective 1a homolog; aph-1 homolog A, gamma secretase subunit; APH-1a; Aph-1alpha; APH1A gamma secretase subunit; Gamma-secretase subunit APH-1A; Presenilin-stabilization factor; RP4-790G17.3, 6530402N02Rik, APH-1, APH-1A, CGI-78

Gene Aliases: 6530402N02Rik; APH-1; APH-1A; APH1A; CGI-78; PSF; RGD1311546; UNQ579/PRO1141

UniProt ID: (Human) Q96BI3, (Mouse) Q8BVF7

Entrez Gene ID: (Human) 51107, (Mouse) 226548, (Rat) 365872

Molecular Function: enzyme modulator