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Invitrogen
APLF Polyclonal Antibody
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FIGURE: 1 / 2
APLF Antibody (PA5-114861) in ICC/IF
Immunocytochemistry analysis of APLF in A549 cells (H2O2 treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with APLF polyclonal antibody (Product # PA5-114861) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-114861
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human APLF(Accession Q8IW19), corresponding to amino acid residues L92-G142.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total APLF.
Target Information
C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks.[supplied by OMIM, Mar 2008].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aprataxin and PNK-like factor; aprataxin- and PNK-like factor; Apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; XRCC1-interacting protein 1; zinc finger, CX5CX6HX5H motif containing 1
Gene Aliases: 2010301N04Rik; AI452191; APFL; APLF; C2orf13; PALF; RGD1565557; XIP1; ZCCHH1
UniProt ID: (Human) Q8IW19, (Mouse) Q9D842
Entrez Gene ID: (Human) 200558, (Rat) 500247, (Mouse) 72103
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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