Invitrogen

APLF Polyclonal Antibody

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APLF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of APLF in paraffin-embedded human brain tissue using an APLF polyclonal antibody (Product # PA5-39776).

APLF Antibody in Western Blot (WB) — Western blot analysis of APLF was performed by loading ~300,000 cell equivalents per lane in 1X LDS reducing sample buffer (Product # NP0007) and molecular weight ladder onto a 3-8% Tris-Acetate protein gel (Product # EA0375BOX). Proteins were transferred to a PVDF membrane at 100 mA overnight. Membrane was blocked in 5% milk/TBST for 1 hour. APLF was detected using a polyclonal antibody (Product # PA5-39776) diluted 1:2000 at 4°C, overnight. Membrane was then probed with anti-rabbit secondary antibody at a dilution of 1:5000. Chemiluminescent detection was performed using Pierce ECL western blot substrate (Product # 32106). Data courtesy of the Antibody Data Exchange Program.

Product Details

PA5-39776

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic non-phosphopeptide derived from human APLF around the phosphorylation site of Ser116 (R-N-SP-Q-V)
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Storage conditions	-20°C
RRID	AB_2556327

Target Information

C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks.[supplied by OMIM, Mar 2008].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PARP2 mediates branched poly ADP-ribosylation in response to DNA damage.

Nature communications

Chen Q,Kassab MA,Dantzer F,Yu X

Published figure using APLF polyclonal antibody (Product # PA5-39776) in Western Blot

Mon Aug 13 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Aprataxin and PNK-like factor; aprataxin- and PNK-like factor; Apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; XRCC1-interacting protein 1; zinc finger, CX5CX6HX5H motif containing 1

Gene Aliases: APLF; C2orf13; PALF; XIP1

APLF Polyclonal Antibody

Product Details

PA5-39776

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage conditions

RRID

Target Information

Bioinformatics

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