Display Info:
cookie.CK_ISO_CODE.value:us
cookie.CK_LANG_CODE.value:en
cookie.CK_DISPLAY_TYPE.value:
pageContext.request:org.apache.struts2.dispatcher.StrutsRequestWrapper@5b0712de
Request attributes: pageContext.request.scheme:http(not used)
syndicationProtocol:https
syndicationExtensionPrefix:
syndicationDisplayType:

Invitrogen

APOE Polyclonal Antibody

View all (38) APOE antibodies
635D49A6-4B57-425E-8DC4-6BA1AEB7B473 Created with sketchtool. Datasheet
433BB748-DC81-4FE4-83AD-384F97313745 Created with sketchtool. Create Citation
C488C313-B559-4BFD-B17D-A8B65492815A Created with sketchtool. Tech Support
635D49A6-4B57-425E-8DC4-6BA1AEB7B473 Created with sketchtool. Datasheet
433BB748-DC81-4FE4-83AD-384F97313745 Created with sketchtool. Create Citation
C488C313-B559-4BFD-B17D-A8B65492815A Created with sketchtool. Tech Support

Product Details

Tested Applications
Dilution
Immunocytochemistry (ICC)
1:100-1:1000
Immunofluorescence (IF)
1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:1000
Immunoprecipitation (IP)
1:100-1:500
Western Blot (WB)
1:500-1:3000
Product Specifications
Species Reactivity
Human
Host / Isotype
Rabbit / IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region within amino acids 255 and 317 of Human Apo E
Conjugate
Unconjugated
Form
Liquid
Concentration
0.66 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% Proclin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2544564

Product Specific Information

PA5-27088 targets Apo E in IHC (P), IP, IF, and WB applications and shows reactivity with Human samples.

The PA5-27088 immunogen is synthetic peptide corresponding to a region within amino acids 255 and 317 of Human Apo E.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AD2; Apo-E; Apolipoprotein E; apolipoprotein E3; LDLCQ5; LPG

Gene Aliases: AD2; APO-E; APOE; LDLCQ5; LPG

UniProt ID: (Human) P02649

Entrez Gene ID: (Human) 348

Function(s)
response to reactive oxygen species retinoid metabolic process negative regulation of endothelial cell proliferation response to dietary excess triglyceride metabolic process cholesterol catabolic process cellular calcium ion homeostasis receptor-mediated endocytosis cytoskeleton organization G-protein coupled receptor signaling pathway nitric oxide mediated signal transduction synaptic transmission, cholinergic cholesterol metabolic process regulation of gene expression negative regulation of platelet activation positive regulation of cholesterol esterification positive regulation of cholesterol efflux long-chain fatty acid transport protein import virion assembly triglyceride catabolic process cGMP-mediated signaling negative regulation of blood coagulation regulation of axon extension positive regulation of cGMP biosynthetic process neuron projection regeneration regulation of Cdc42 protein signal transduction positive regulation of low-density lipoprotein particle receptor catabolic process cholesterol efflux phospholipid efflux very-low-density lipoprotein particle remodeling low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly chylomicron remnant clearance high-density lipoprotein particle clearance very-low-density lipoprotein particle clearance lipoprotein metabolic process lipoprotein biosynthetic process lipoprotein catabolic process vasodilation cholesterol homeostasis negative regulation of MAP kinase activity negative regulation of neuron apoptotic process negative regulation of blood vessel endothelial cell migration reverse cholesterol transport positive regulation by host of viral process negative regulation of cholesterol biosynthetic process positive regulation of lipid biosynthetic process intracellular transport regulation of neuronal synaptic plasticity artery morphogenesis negative regulation of inflammatory response positive regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis maintenance of location in cell fatty acid homeostasis positive regulation of dendritic spine development negative regulation of canonical Wnt signaling pathway AMPA glutamate receptor clustering NMDA glutamate receptor clustering cellular oxidant detoxification regulation of beta-amyloid clearance negative regulation of neuron death positive regulation of postsynaptic membrane organization negative regulation of presynaptic membrane organization negative regulation of beta-amyloid formation positive regulation of dendritic spine maintenance positive regulation of phospholipid efflux positive regulation of lipid transport across blood brain barrier
Process(es)
beta-amyloid binding lipid transporter activity protein binding phospholipid binding heparin binding lipid binding cholesterol binding antioxidant activity cholesterol transporter activity identical protein binding protein homodimerization activity metal chelating activity tau protein binding low-density lipoprotein particle receptor binding phosphatidylcholine-sterol O-acyltransferase activator activity very-low-density lipoprotein particle receptor binding lipoprotein particle binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support
Display Info:
cookie.CK_ISO_CODE.value:us
cookie.CK_LANG_CODE.value:en
cookie.CK_DISPLAY_TYPE.value:
pageContext.request.scheme:http(not used)
syndicationProtocol:https
syndicationExtensionPrefix:
syndicationDisplayType:

 

page info