Invitrogen

APOE Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

3 References

View all (49) APOE antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

APOE Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of APOE was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with APOE Rabbit Polyclonal Antibody (Product # PA5-27088) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of APOE was performed in paraffin-embedded human ovarian cancer tissue using APOE Polyclonal Antibody (Product # PA5-27088) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

APOE Antibody in Western Blot (WB) — Western blot was performed using Anti-APOE Polyclonal Antibody (Product # PA5-27088) and a 36 kDa band corresponding to APOE was observed in Hep G2 with PTI, Brefeldin treatment and Caco-2 with PTI treatment but was absent in A-431 and Daudi which are reported to be negative. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with Protein Transport Inhibitor (PTI; 1x for 4 hours) (Lane 2), Hep G2 treated with Brefeldin (10ng/mL for 90 mins) (Lane 3) Caco-2 (Lane 4), Caco-2 treated with Protein Transport Inhibitor (PTI; 1x for 4 hours) (Lane 5), A-431 (Lane 6), A-431 treated with Protein Transport Inhibitor (PTI; 1x for 4 hours) (Lane 7), Daudi (Lane 8) and Daudi treated with Protein Transport Inhibitor (1x for 4 hours) (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:3000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

APOE Antibody in Immunoprecipitation (IP) — APOE Polyclonal Antibody immunoprecipitates Apolipoprotein E protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract. A : 30 µg whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cells. B : Control with 3 µg of pre-immune rabbit IgG. C : Immunoprecipitation of Apolipoprotein E by 3 µg of APOE Polyclonal Antibody (Product # PA5-27088). 10% SDS-PAGE. The immunoprecipitated Apolipoprotein E protein was detected by APOE Polyclonal Antibody (Product # PA5-27088) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.

APOE Antibody in ELISA (ELISA) — Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using APOE Polyclonal Antibody (Product # PA5-27088) as capture antibody at concentration of 5 µg/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) was diluted at 1:10,000 and used to detect the primary antibody.

APOE Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in APOE (KO) cell line compared to control cell line using Anti-ApoE Polyclonal Antibody (Product # PA5-27088).

Product Details

PA5-27088

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

ELISA (ELISA)

1:1,000-1:10,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 255 and 317 of Human Apo E

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.66 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544564

Product Specific Information

Recommended positive controls: HepG2, Conditional medium from differentiated human primary preadipocyte, human plasma.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Plaque-associated human microglia accumulate lipid droplets in a chimeric model of Alzheimer's disease.

Molecular neurodegeneration

Claes C,Danhash EP,Hasselmann J,Chadarevian JP,Shabestari SK,England WE,Lim TE,Hidalgo JLS,Spitale RC,Davtyan H,Blurton-Jones M

PA5-27088 was used in Immunohistochemistry (Frozen) to indicate lipid droplet accumulation occurs in human DAM xMGs in AD, but is reduced in TREM2-R47H DAM xMGs, as it occurs secondary to TREM2-mediated changes in plaque proximity and reactivity.

Fri Jul 23 00:00:00 EDT 2021

Morphological analysis of Apolipoprotein E binding to Aβ Amyloid using a combination of Surface Plasmon Resonance, Immunogold Labeling and Scanning Electron Microscopy.

BMC biotechnology

Islam T,Gharibyan AL,Lee CC,Olofsson A

Published figure using APOE polyclonal antibody (Product # PA5-27088) in Immunocytochemistry

Wed Dec 11 00:00:00 EST 2019

Complement-Mediated Regulation of Apolipoprotein E in Cultured Human RPE Cells.

Investigative ophthalmology & visual science

Yang P,Skiba NP,Tewkesbury GM,Treboschi VM,Baciu P,Jaffe GJ

PA5-27088 was used in Immunocytochemistry to study the effect of complement activation on induction of apolipoprotein E accumulation in human retinal pigment epithelial cells.

Thu Jun 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Apo-E; ApoE4; Apolipoprotein; Apolipoprotein E; apolipoprotein E3; apolipoprotein E4; Apoprotein; B2G1; BG; MGC1571

Gene Aliases: AD2; APO-E; APOE; LDLCQ5; LPG

UniProt ID: (Human) P02649

Entrez Gene ID: (Human) 348

Molecular Function: apolipoprotein

Function(s)

Process(es)

beta-amyloid binding lipid transporter activity protein binding phospholipid binding heparin binding lipid binding cholesterol binding antioxidant activity cholesterol transporter activity identical protein binding protein homodimerization activity metal chelating activity tau protein binding low-density lipoprotein particle receptor binding phosphatidylcholine-sterol O-acyltransferase activator activity very-low-density lipoprotein particle receptor binding lipoprotein particle binding